1997 Fiscal Year Final Research Report Summary
Development of mechanical device to continuously guantitute platelet thrunbus formation under flow conditions.
| Project/Area Number |
07557079
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Hematology
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| Research Institution | Keio University School of Medicine |
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Principal Investigator |
IKEDA Yasuo Keio University School of Medicine Internal Medicine Professor, 医学部, 教授 (00110883)
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| Co-Investigator(Kenkyū-buntansha) |
ODA Atsushi Keio University School of Medicine Internal Medicine, Instructor, 医学部, 助手 (50255436)
MURATA Mitsuru Keio University School of Medicine Internal Medicine, Assist.Professor, 医学部, 講師 (50174305)
HANDA Makoto Keio University School of Medicine Internal Medicine, Assist.Professor, 医学部, 講師 (40129614)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Platelet adhesion / Wall shear rate / Hematocrit / Platelet membrane glycoproteins / Adhesion molecules / Anti-platelet agents |
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| Research Abstract |
Platelet adhesion to subendothelium, one of the earliest steps in primary hemostasis and pathological thrombus formation is followed by subsequent platelet plug formation including platelet-platelet interactions. Therefore, it is difficult to seggregate from the subsequent platelet interactions and analyze the mechanism of platelet adhesion only. We developed a recirculating flow system including a quartz crystal microbalance (QCM) as a sensor which allows continuous monitoring of the surface coverage of firmly adherent platelets only. Using this system, we found that platelet adhesions to collagen (bovine tendon type 1 : CL) and human von Willebrand factor (vWf) were dependent upon wall shear rate (310/s-2,400/s) and hematocrit (20-45%). It was found that there existed two recptor-ligand pairs i.e. platelet glycoprotein (GP) Ib/IX-vWf and GPIa/IIa-CL independently operating in platelet adhesion to CL evenunder higher flow conditions. Interestingly, well-known anti-platelet agents such as aspirin and cilostazol were found to enhance platelet adhesion to CL,although they readily inhibited subsequent platelet-platelet interactions. Thus, our newly-developed QCM system may be useful to study the mechanism of platelet adhesion under flow conditions and to screen the efficacy of new anti-platelet adhesion drugs.
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