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1997 Fiscal Year Final Research Report Summary

Development of mechanical device to continuously guantitute platelet thrunbus formation under flow conditions.

Research Project

Project/Area Number 07557079
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field Hematology
Research InstitutionKeio University School of Medicine

Principal Investigator

IKEDA Yasuo  Keio University School of Medicine Internal Medicine Professor, 医学部, 教授 (00110883)

Co-Investigator(Kenkyū-buntansha) ODA Atsushi  Keio University School of Medicine Internal Medicine, Instructor, 医学部, 助手 (50255436)
MURATA Mitsuru  Keio University School of Medicine Internal Medicine, Assist.Professor, 医学部, 講師 (50174305)
HANDA Makoto  Keio University School of Medicine Internal Medicine, Assist.Professor, 医学部, 講師 (40129614)
Project Period (FY) 1995 – 1997
KeywordsPlatelet adhesion / Wall shear rate / Hematocrit / Platelet membrane glycoproteins / Adhesion molecules / Anti-platelet agents
Research Abstract

Platelet adhesion to subendothelium, one of the earliest steps in primary hemostasis and pathological thrombus formation is followed by subsequent platelet plug formation including platelet-platelet interactions. Therefore, it is difficult to seggregate from the subsequent platelet interactions and analyze the mechanism of platelet adhesion only. We developed a recirculating flow system including a quartz crystal microbalance (QCM) as a sensor which allows continuous monitoring of the surface coverage of firmly adherent platelets only. Using this system, we found that platelet adhesions to collagen (bovine tendon type 1 : CL) and human von Willebrand factor (vWf) were dependent upon wall shear rate (310/s-2,400/s) and hematocrit (20-45%). It was found that there existed two recptor-ligand pairs i.e. platelet glycoprotein (GP) Ib/IX-vWf and GPIa/IIa-CL independently operating in platelet adhesion to CL evenunder higher flow conditions. Interestingly, well-known anti-platelet agents such as aspirin and cilostazol were found to enhance platelet adhesion to CL,although they readily inhibited subsequent platelet-platelet interactions. Thus, our newly-developed QCM system may be useful to study the mechanism of platelet adhesion under flow conditions and to screen the efficacy of new anti-platelet adhesion drugs.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kameda H, Morita I, Handa M., Ikeda Yetal: "Re-expression of functional P-selectin molecules on the endothelial cell surface by repeated stimulation with thrombin." Brit J Haematol 97. 97. 348-355 (1997.)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Goto S., Sakai H., Ikeda Y, and Handa S.:"Acute myocardial infarction plasma augments platelet thrombus growth in high shear rates." The Lancet. 349(9051). 543-544 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoyama K., Handa M., and Ikeda Y.et al: "Characterization of the novel murine monoclonal anti-von Willebrand factor(vWf)antibody GUR76-23 which inhibits vWf interaction with α IIb β 3 but not αvβ3 integrin." Biochem Biophys Res Commun. 234. 147-152 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iijima K, Murata M., Handa M., Ikeda Y.et al: "High shear stress attenuates agonist-induced,glycoprotein IIb/IIIa-mediated platelet aggregation when von Willebrand factor binding to glycoprotein Ib/IX is blocked." Biochem Biophys Res Commun. 233(3). 796-800 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyakawa Y., Oda A., Handa M., Ikeda Y.et al: "Thrombopoietin and thrombin induce tyrosine phosphorylation of vav in human blood platelets." Blood. 89(8). 2789-2798 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikeda Y., Murata M., and Goto S.: "von Willebrand factor-dependent shear-induced platelet aggregation:basic mechanisms and clinical implications." The Annals of the New York Academy of Sciences. 811. 325-336 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Moriki T., Murata M., Handa M., Ikeda Y.: "Expression and functional characterization of an abnormal platelet membrane glycoprotein Ib α(Met239→Val)reported in patients with platelet-type von Willebrand disease." Blood. 89(10). 3565-3573 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kameda H., Morita I., Handa M., Ikeda Y et al: "Re-expression of functional P-selectin molecules on the endothelial cell surface by repeated stimulation with thrombin." Brit J Haematol. 97. 348-355 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Goto S., Sakai H., Ikeda Y,and Handa S.: "Acute myocardial infarction plasma augments platelet thrombus growth in high shear rates." The Lancet. 349(9051). 543-544 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoyama K., Handa M., Oda A.and Ikeda Y.et al: "Characterization of the novel murine monoclonal anti-von Willebrand factor (vWf) antibody GUR76-23 which inhibits vWf interaction with alpha IIb beta 3 but not alpha v beta 3 integrin." Biochem Biophys Res Commun. 234. 147-152 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iijima K., Murata M., Handa M., Ikeda Y.et al: "High shear stress attenuates agonist-induced, glycoprotein IIb/IIIamediated platelet aggregation when von Willebrand factor binding to glycoprotein Ib/IX is blocked." Biochem Biophys Res Commun. 233(3). 796-800 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyakawa Y., Oda A., Handa M., Ikeda Y.et al: "Thrombopoietin and thrombin induce tyrosine phosphorylation of vav in human blood platelets." Blood. 89(8). 2789-2798 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda Y., Murata M., and Goto S.: "von Willebrand factor-dependent shear-induced platelet aggregation : basic mechanisms and clinical implications." The Annals of the New York Academy of Sciences. 811. 325-336 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Moriki T., Murata M., Handa M., Ikeda Y.: "Expression and functional characterization of an abnormal platelet membrane glycoprotein Ib alpha (Met239*Val) reported in patients with platelet-type von Willebrand disease." Blood. 89(10). 3565-3573 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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