1997 Fiscal Year Final Research Report Summary
Generation of Cultured Cell Lines to Study Alzheimer's Disease
| Project/Area Number |
07557165
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
医薬分子機能学
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| Research Institution | The University of Tokushima |
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Principal Investigator |
YAMAUTI Takasi Faculty of Pharm.sci., The University of Tokushima Professor, 薬学部, 教授 (90041813)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Al_3 heimer's disese / Ca^<2+> / calmoclulin / tau protcin / phophorylition / protein kinase / clmed cell line |
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| Research Abstract |
Alzheimer's disease (AD) is the most common neurodegenerative disorder worldwide, causing progressive dementia and death in many millions of people. Neurofibrillary tangles are characteristic lesions found in the brain of AD.These tangles are composed of paired helical filaments (PHFs), and the major component is the microtubule-associated protein tau. PHF-tau is abnormally hyperphosphorylated in AD.In order to investigate the control of phosphorylation of tau protein, we studied in cultured cells and obtained some interesting results as follows ; (i) We constructed the expression vector of tau protein and protein kinases, and transfected them to neuroblastoma cells or embryonic carcinoma cells (cell line P-19). Cloned cells expressing tau protein or protein kinases were generated and isolated. (ii) Treatment of retinoic acid on P-19 cells increased the expression of CaM kinase II and induced to change the splicing pattern of the delta isoform of the kinase. (iii) Neurite outgrowth was stimulated in cells overexpressing CaM kinase II with protein kinase C inhibitors, indicating that differentiation and proliferation of neural cells were regulated by the activity of protein kinase C and CaM kinase II.
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Research Products
(12 results)
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[Publications] Ogawara, M., Inagaki, N., Tsujimura, K., Takai, Y., Sekimata, M., Ha, M.H., Imajoh-Ohmi, S., Hirai, S., Ohno, S., Sugiura, H., Yamauchi, T., and Inagaki, M.: "Differential targeting of protein kinase C and CaM kinase II signalings to vimentin." J.Cell Biol.131. 1055-1066 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Nishioka, N., Shiojiri, M., Kadota, S., Morinaga, H., Kuwahara, J., Arakawa, T., Yamamoto, S., and Yamauchi, T.: "Gene of rat Ca^<2+>/calmodulin-dependent protein kinase II alpha isoform -its cloning and whole structure." FEBS Lett.396. 333-336 (1996)
Description
「研究成果報告書概要(欧文)」より
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