1996 Fiscal Year Final Research Report Summary
TRIAL OF SEARCHING FOR MOTORNEURON-SPECIFIC GENES
| Project/Area Number |
07557340
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 試験 |
|---|
| Research Field |
Neurology
|
|---|
| Research Institution | TOKYO MEDICAL AND DENTAL UNIVERSITY |
|---|
Principal Investigator |
YAMADA Masahito TOKYO MEDICAL AND DENTAL UNIVERSITY,SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (80191336)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MICHIKAWA Makoto TOKYO MEDICAL AND DENTAL UNIVERSITY,SCHOOL OF MEDICINE,ASSISTANT, 医学部, 助手 (40270912)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Keywords | MOTORNEURON / SUBTRACTION cDNA LIBRARY / THROMOSPONDIN / NEURAL DEVELOPMENT |
|---|
| Research Abstract |
To isolate genes specifically expressing motor neurons, we constructed subtracton cDNA library using mouse motor neuron x neuroblastoma hybrid cell line, NSC-19. We screened 200 clones of the subtraction cDNA library and isolated three clones specifically expressing to this cell line. One of these was identified as amyloid precursor like protein 1, and the other two clones were both novel genes. One of novel genes was expressing in the central nervous system during embryonal stage. We obtained cDNAs containing full-length open reading frame and found that its deduced amino acids encoded a novel secretary glycoprotein containing thrombospondin (TSP) type 1 domain. Investigaton of embryonal expression by in situ hybridization revealed that this gene expressed predominantly in some parts of telecephalon, roof plates of midbrain and spinal cord, and a part of limb buds. Expression in central nervous system (CNS) is especially localized to dorsal part of neural tube, implicating of important association of this gene with developmental process in dorsal part of neural tube. Proteins with TSP type 1 domain, especially associating with CNS,includes thrombospondin, F-spondin, SCO-spondin, and UNC-5 TSP type 1 domain is considered to have functions such as cell adhesion and neurite growth. Therefore, this novel gene may be associated with organogenesis or neuronal development in dorsal part of neural tube. We consider the necessity of further investigation about the relationship of this gene and motor neuron.
|
