1996 Fiscal Year Final Research Report Summary
Development of SSLP markers in rat models for diabetes and hypertension.
| Project/Area Number |
07557352
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
内分泌・代謝学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SERIKAWA Tadao Kyoto University, Institute of Laboratory Animals, Professor, 医学研究科, 教授 (30025655)
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| Co-Investigator(Kenkyū-buntansha) |
KOMEDA Kajyuro Tokyo Medical College, Animal Research Center, Associate Professor, 医学部, 助教授 (90074533)
OGIWARA Toshio Osaka University Medical School, Geriatric Medicine, Professor, 医学部, 教授 (60107042)
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| Project Period (FY) |
1995 – 1996
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| Keywords | rat / microsatellite marker / SSLP marker / hypertinsion / diabetes / genetic analysis / animal model / gene mapping |
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| Research Abstract |
The rat has been widely used as an experimental animal model for many human diseases. They are particularly useful in the study of multifactorial diseases including hypertension and diabetes. To identify genetic loci involved in the pathogenesis of these types of complex diseases, it is necessary to develop detailed genetic map. Therefore, we developed rat SSLP markers in this study. In addition, we mapped disease-related loci in rat models for diabetes or hypertension.
1.Rat SSLP markers
We developed 20 polymorphic DNA markers (13 anonymous DNA fragments and 7 rat genes). In addition, we evaluated the PCR primer sets which were supplied from Research Genetics, and mapped 56 new loci in the rat genetic map. Using mouse SSLP primers, we mapped 20SSLP markers in the rat.
2.Genetic analysis of rat models for diabetes
Genetic analysis of BB/OK rat (a model for type 1 diabetes) suggested that the third gene for susceptibility of diabetes is located near Olf locus (Chr.18). In the KDP rat, a new model for type 1 diabetes, we mapped a major diabetes-susceptible gene (Iddm/kdp1) on the rat chromosome 11. The mapped region was found to be conserved on mouse choromosome 16 and human choromosome 3.
3.Genetic analysis of rat models for hypertension
Genetic analysis of SER indicated that Cryg locus (Chr.9) were cosegragated with high blood pressure. Pkata locus (Chr.8) was cosegregated with decreased blood pressure in SER and DIS/Eis (Dahl rat). In addition, Gja1 locus(Chr 18) cosegeregated with body weight in SHR.
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