1996 Fiscal Year Final Research Report Summary
Development of chemokine-expressing retrovirus vector system in malignant gliomas
| Project/Area Number |
07557359
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | KANAZAWA UNIVERSITY |
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Principal Investigator |
YAMASHITA Junkoh Kanazawa University School of Medicine, 医学部, 教授 (90026948)
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| Co-Investigator(Kenkyū-buntansha) |
AMANUMA Hiroshi Tsukuba Life Science Cente The Institute of Physical and Chemical Research (RIKE, 理化学研究所, 主任研究員
YAMAGUCHI Kazuo Kanazawa University, Institute for Gene Research Director and Professor, 遺伝子実験施設, 施設長教授 (00019879)
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| Project Period (FY) |
1995 – 1996
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| Keywords | chemokine / MIP-1alpha / MCAF / retrovirus vector / adenovirus vector / glioma |
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| Research Abstract |
The C-C chemokines including such molecules as MIP-1alpha (macrophage inflammatory protein-1 alpha) and MCAF (monocyte chemotactic and activity factor) exhibit chemoattractant potential for monocytes and T lymphocytes. We have developed retrovirus and adenovirus vector system which allow expression of the C-C chemokines to evaluate the effect of localized secretion of the chemokines on host anti-tumor response in rat given subcutaneous innoculation of 9L gliosarcoma cells followed by virus injection in vivio. The production of MIP-1alpha by infected adenovirus did not influence the growth rate in vitro, but suppressed tumor growth when the virus is injected into 9L flank tumor of F344 rats. Histological examination of the site of injection of 9L injected with MIP-1alpha expressing adenovirus vector showed predominantly T cell infiltration. These results indicated that chemokines have potent anti-tumor activities when released at the tumor site. Our studies highlight the potential usefulness of localized chemokine secretion in inducing potent antitumor host responses.
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Research Products
(19 results)
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[Publications] Yamashima T,Saido T,Takita M,Miyazawa A,Yamano J,Miyakawa A Nishijo H,Yamashita J,Kawashima S,Ono T,Yoshioka T.: "Transient brain ischemia provokes Ca2+, PIP2 and calpain responses prior to delayd neuronal death in monkeys" Eur J Neurosci. 8. 1932-1944 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Sakuda K,Kohda Y,Matsumoto T,Park C,Seto A,Tohma Y,Hasegawa M,Kida S,Nitta H,Yamashima T,Yamashita J: "Expression of NF2 gene product merlin in arachnoid villi and meningiomas" Brain Tumor Pathol. 13. 145-148 (1996)
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「研究成果報告書概要(欧文)」より
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