1996 Fiscal Year Final Research Report Summary
Determination and regulation of half-life of the proteins
| Project/Area Number |
07558094
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 試験 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | University of Tokyo |
|---|
Principal Investigator |
SUZUKI Koichi UNIV.OF TOKYO,PROFESSOR, 分子細胞生物学研究所, 教授 (80011948)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SORIMACHI Hiroyuki UNIV.OF TOKYO,ASSIST.PROFESSOR, 分子細胞生物学研究所, 助手 (10211327)
SAIDO Takaomi TOKYO MET.INST.MED., RESEARCH ASSOCIATE, 研究員 (80205690)
ISHIURA Shoichi UNIV.OF TOKYO,ASSOCIATE PROFESSOR, 分子細胞生物学研究所, 助教授 (10158743)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Keywords | PEOTEIN DEGRADATION / CALCIUM / HALF-LIFE / MUSCULAR DYSTROPHY |
|---|
| Research Abstract |
It is now clear that member of the calpain (calcium-activated neutral protease) family play key biological roles in down-regulation of key signal molecules and mammalian intracellular protein degradation. In chicken, we identified three conventional isoforms, m-, m/m- and m-calpains, according to cDNA cloning followed by expression in baculovirus and purification of the enzymes. We speculate that each of these enzymes is synthesized as an inactive proenzyme (80 kDa plus 30 kDa) that is proteolytically activated to form an 80 kDa monomeric catalytic enzyme.
Hereditary Limb-Girdle muscular dystrophy type 2A (LGMD2A) is a rare genetic disorder characterized by muscle atrophy. Several missence mutations of the muscle specific calpain, p94, gene have been reported to be associated with the LGMD2A phenotype. We found that p94 is attached to muscle elastic protein connectin and the mutation of the p94 gene disrupted the association of the enzyme with connectin. Loss of autolytic activity of p94 in these LGMD2A patients would permit both inhibition of autodigestion and dissociation from muscle structure resulting in muscle atrophy.
|
Research Products
(16 results)
-
-
-
-
-
-
-
-
[Publications] Sorimachi,H.,Kinbara,K.,Kimura,S.,Takahashi,M.,et al: "Muscle-specific calpain,p94,responsible for limb girdle muscular dystrophy type 2A,associates with connectin through IS2,a p94-specific sequence IS2." J.Biol.Chem.270. 31158-3116 (1996)
Description
「研究成果報告書概要(和文)」より
-
-
[Publications] Shimokawa, M., Ishiura, S., Kameda, N., Yamamoto, M., Sasagawa, N., Saitoh, N., Sorimachi, H., Ueda, H., Ohno, S., Suzuki, K.& Kobayashi, T.: "A novel isoform of myotonin protein kinase : Gene product of myotonic dystrophy is localized in the sarcoplasmic reticulum of skeketal muscle." Am. J.Pathol.(in press). (1997)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Sasagawa, N., Saitoh, N., Shimokawa, M., Sorimachi, H., Maruyama, K., Arahata, K., Ishiura, S.& Suzuki, K.: "Effect of artificial CTG repeat expansion on the expression of myotonin protein kinase in COS-1 cells." Biochim. Biophys. Acta. 1315. 112-116 (1996)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Maruyama, K., Tomita, T., Shinozaki, K., Kume, H., Asada, H., Saido, S.C., Ishiura, S., Iwatsubo, T.& Obata, K.: "Familial Alzheimer's disease-linked mutations at Va1717 of amyloid precursor protein are specific for the increased secretion of Abeta42 (43)." Biochem. Biophys. Res. Commun.227. 730-735 (1996)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Yazaki, M., Tagawa, K., Maruyama, K., Tsuchiya, T., Ishiura, S & Suzuki, K.: "Mutation of potential N-linked glycosylation sites in the Alzheimer's disease amyloid precursor protein." Neurosci. Lett.221. 57-60 (1996)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Sorimachi, H., Forsberg, N., Lee, H.J., Joeng, S.Y., Richard, I., Beckmann, J.S., Ishiura, S.& Suzuki, K.: "Highly conserved structure in the promoter region of the gene for muscle specific calpain, p94." Biol. Chem. Hoppe-Seyler. 377. 859-864 (1996)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Sorimachi, H., Kinbara, K., Kimura, S., Takahashi, M., Ishiura, S., Sasagawa, N., Sorimachi, N., Shimada, H., Tagawa, K., Maruyama, K.& Suzuki, K.: "Muscle-specific calpain, p94, responsible for limb girdle muscular dystrophy type 2A,associates with connectin through IS2, a p94-specific sequence IS2." J.Biol. Chem.270. 31158-3116 (1996)
Description
「研究成果報告書概要(欧文)」より
