1997 Fiscal Year Final Research Report Summary
Establishment of Mouse Consomic Lines
| Project/Area Number |
07558116
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Laboratory animal science
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
SHIROISHI Toshihiko National Institute of Genetics, Mammal.Genet.Lab., Asoc.Prof., 系統生物研究センター, 助教授 (90171058)
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| Co-Investigator(Kenkyū-buntansha) |
WAKANA Shigeharu Central Inst.Exp.Animals, Gene Analysis Lab., Lab.Chief, DNA解析室, 研究員 (90192434)
YONEKAWA Hiromichi Tokyo Metropol.Inst.Med.Sci.Exp.Animal Lab., Lab.Head, 実験動紡研究室, 部長 (30142110)
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| Project Period (FY) |
1995 – 1997
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| Keywords | mouse / subspecies / chromosome / backcross / microsatellite / consomic / linkage |
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| Research Abstract |
From the definition, two strains of mouse are referred to as consomic when they differ by one complete chromosome pair. Given that mouse has a karyotype with 21 different chromosomes (19 autosomes + X,Y chromosomes), 21 consomic mouse strains are required for full coverage of mouse chromosomes by donor strains, from which one complate chromosome is introduced into the common genetic background referred to as recipient. Chromosome substitution is achieved by six to seven times backcrossing of individuals identified as heterozygous for the selected chromosome to the recipient strain.
It has been well established that many pathological conditions and diseases like cancer and diabetes are under multigenic control. They are generally difficult to analyze when a great number of genes are involved and epistatic interactions are present among the genes. In these cases, if a phenotype of interest appears to deviate in a consomic strain from what is known in the genetic background strain, its genetic control must be related to chromosomal substitution to be concerned. Further genetic analysis will then be considerably simplified.
We started to establish consomic strains, using. MSM strain, which is derived from Japanese wild mouse, Mus musculus molossinus, as donor strain and a C57BL/6J laboratory strain as recipient. These two strains are genetically very remote from each other so that they differ in a number of genetic traits. This genetic defference faclitates in chromosomal assignment of any phenotype to be examined and is of great use in linkage analysis based on polymorphism of DNA markers. We used microsatellite DNA markers, at least five markers per chromosome, for selection of individuals in each step of backcrossing. As a result, we have obtained 15 consomic strains which carry MSM derived chromosomes in the genetic background of C57BL/6J strain.
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Research Products
(12 results)
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[Publications] Ohtsuka, H., Oyanagi, M., Mafune, Y., Miyashita, N., Shiroishi, T., Moriwaki, K., Kominami, R.and Saitou, N.: "The presence/absence polymorphism and evolution of the p653 pseudogene in the genus Mus." Phylogenet.Evol.5. 548-556 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Wakabayashi, Y., Kikkawa, Y., Matsumoto, Y., Shinbo, T., Kosugi, S., Chou D., Furuya, M., Jishage, K., Noda, T., Yonekawa, H.and Kominami, R.: "Genetic and physical delineation of the region of the mouse dearness mutation shaker-2." Biochem. Biophys. Res. Commun.234. 107-110 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Shiroishi, T., Ueda, Y., Ball, S., Nagamine, C.M., Sagai, T and Moriwaki, K.: "Genetic differentiation of mouse Y chromosome." J.Repro. Dev.43, Supplement. 17-18 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Koide, T., Moriwaki, K., Uchida, K., Mita, A., Sagai, T., Yonekawa, H., Katoh, H., Miyashita, N., Tsuchiya, K., Nielsen, T.J.and Shiroishi, T.: "A new inbred strain JF1 established from Japanese fancy mouse carrying the classical piebald allele." Mammal.Genome. 9. 15-19 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sato, H., Koide, T., Masuya, H., Wakana, S., Sagai, T., Ishiguro, S., Tamai, M.and Shiroishi, T.: "A new mutation Rim3 resembling Reden is mapped close to reinoic acid receprot alpha (Rara) gene on mouse chromosome 11." Mammal. Genome.9. 20-25 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sugai, T., Koide, T., Endo, M., Tanoue, K., Kikkawa, H., Yonekawa, H., Ishiguro, S., Tanai, S., Matsuda, Y., Wakana, S.and Shiroishi, T.: "rim2 (recombination induced mutation 2) is a new allele of pearl and a mouse model of human Hermansky-Pudlak syndrom (HPS) : genetic and physical mapping." Mammal. Genome.9. 2-7 (1998)
Description
「研究成果報告書概要(欧文)」より
