1996 Fiscal Year Final Research Report Summary
Development of a Culture System of Living Tissues under Dynamic Load Condition
| Project/Area Number |
07558124
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Osaka University |
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Principal Investigator |
HAYASHI Kozaburo Osaka Univ., Fac.of Eng.Sci., Professor, 基礎工学部, 教授 (90026196)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAKI Hiroshi Osaka Univ., Fac.of Eng.Sci., Research Associate, 基礎工学部, 助手 (00263228)
FUJIE Hiromichi Osaka Univ., Fac.of Eng.Sci., Associate Professor, 基礎工学部, 助教授 (20199300)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Living Tissue / Tissue Culture / Dynamic Loading / Collagen Fascicle / Remodeling / Biomechanics |
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| Research Abstract |
1.To study the function of living tissues cultured under various mechanical loading conditions, a system was designed for the application of dynamic load to cultured tissues, which consists of a tissue culture chamber, a linear actuator, and a load cell. The temperature in the culture chamber is maintained at 37ﾟC with heated water circulation, and pH of the medium is maintained with a mixture of 95% air and 5% CO_2 gas.
2.To evaluate the performance of the system, collagen fascicles obtained from a rabbit patellar tendon were cultured under static load condition. The viability of fibroblasts in the fascicles was maintained at the level of non-cultured, control fascicles for 2 weeks.
3.The material of culture dish and grips were changed from polymethylmethacrylate to polycarbonate for sterilization in an autoclave.
4.Collagen fascicles were cultured under static load condition for 1 and 2 weeks, and their mechanical properties were determined. Tensile strength and tangent modulus of collagen fascicles cultured under static load condition for 2 weeks were similar to those of non-cultured, control fascicles. A significant correlation was observed in cultured collagen fascicles between accumulated applied stress and tensile strength.
5.Collagen fascicles were, then, cultured under cyclic load condition for 1 and 2 weeks. The tensile strength and tangent modulus of the fascicles cultured under the cyclic tensile load of 50% of in vivo peak stress were almost the same as those of control fascicles.
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