1996 Fiscal Year Final Research Report Summary
Measurement System of Endothelium-Derived Nitric Oxide Release Induced by Blood Flow
| Project/Area Number |
07558260
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
OGASAWARA Yasuo Kawasaki Medical School, Medical Engineering, Assistant Professor, 医学部, 講師 (10152365)
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| Co-Investigator(Kenkyū-buntansha) |
MOCHIZUKI Seiichi Kawasaki College of Allied Health Professions, Medical Electronics, Assistant Pr, 医用電子技術科, 講師 (60259596)
TSUJIOKA Katsuhiko Kawasaki Medical School, Physiolgy, Professor, 医学部, 教授 (30163801)
KAJIYA Fumihiko Kawasaki Medical School, Medical Engineering, Professor, 医学部, 教授 (70029114)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Endothelium-Derived Relaxing Factor / Nitric Oxide (NO) / Microelectrode / Shear Stress |
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| Research Abstract |
We have attempted to directly measure Nitric Oxide (NO) concentration in the arterial vascular wall, which is induced by blood flow (wall shear stress) using an NO sensing electrode. The NO microelectrode (100 mum in dimeter) was inserted into the vascular media of isolated canine femoral arteries under observation of a light microscope using a micro manipulator. The vessel was then perfused with a Krebs-Henseleit solution, and the flow-related change in NO production was measured by altering perfusion rate. NO concentration in the vascular media increased linearly with increasing perfusion rate (n=7, r^2=0.90-0.99, p<0.02). NO production was increased by perfusing a solution containing L-arginine and was attenuated by N^G--monomethyl-L-arginine (L-NMMA) and N^G-nitro-L-arginine (L-NA), indicating that the NO microelectrode measures NO selectively. We thus confirmed that the concentration of NO in the arterial vascular media changes with changing perfusion rate.
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