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1997 Fiscal Year Final Research Report Summary

Developing Research on Monitoring System of Chemicals Using Bioassay

Research Project

Project/Area Number 07558289
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field 環境影響評価(含放射線生物学)
Research InstitutionOkayama University

Principal Investigator

AOYAMA Isao  Research Institute for Bioresources, Okayama Unviversity Professor, 資源生物科学研究所, 教授 (10026239)

Co-Investigator(Kenkyū-buntansha) RONG Lou  Research Institute for Bioresources, Okayama Unviversity Research Associate, 資源生物科学研究所, 助手 (10284093)
OKAMURA Hideo  Research Institute for Bioresources, Okayama Unviversity Research Associate, 資源生物科学研究所, 助手 (90253020)
Project Period (FY) 1995 – 1997
Keywordstoxic chemicals / bioassay / monitoring / microorganisms / Daphniamagna / lettus / mobility analysis
Research Abstract

It is now anxious that environmental pollution by toxic chemicals is concerned with survival of ecosystem and human begins. Under these circumastances, It is now needed that new detecting systems of toxic chemicals in environments with rapidity, easiness and low cost will be done. The object of this study is to detect the toxic chemical in water and in sediments and to know the strength of their toxicity.
In this study, microorganisms, Daphnia magna, phytoplankton and yeast and seed of Lettus were used for toxicity testing and relative growth rate, biomass after given time, the length of tag time were evaluated as an end points. Not only 24 hours lethality but also early mobility change of Daphnia were evaluated. The mobility change of Daphnia at early time was newly developed as an end point. This is very sensitive index to know the existence of toxic chemicals in water and this method has a rapidity to detect toxicity. It takes only three hours. We investigated the possibility of moni … More toring system to detect toxic chemicals in real environment using these bioassays. The environmental water which we took and investigated were river water. lake water and their sediments and land fill leachates of industrial wastes. Our newly developed bioassay, the method of early mobility analysis of Daphnia takes only three hours. This could be reduced very much the bioassay time compared from normal method which is recommended by OECD which takes from 24 or 48 hours. The sensitivity of this method is found that it is very sensitive to chemicals. When Daphnia is exposed to chemicals a low concentrations, the mobility of Daphnia is stimulated and when is at high concentrations, mobility is inhibited. We found that it can be used as an endpoint expressed as TOXICITY UNIT.
We took environmental samples (water and sediments) along a river and a lake and performed toxicity testing on them. The seasonal change of toxicity was found and suggested that it reflected the agricultural works. The toxicity of land fill leachate was very strong. Only 3% solution of the leachate was detected to be toxicity for Daphnia mobility tests. Daphnia mobility test and elongation inhibition of lettus seeds were more sensitive than micro organisms. Using these battery tests, we can evaluate the ecotoxicity. Less

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 青山 勲: "ミジンコの運動量解析による有害化学物質のモニタリング" 環境技術. 26(4). 255-259 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 青山 勲: "化学物質と生態系" 化学と工業. 50(4). 549-551 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Liu, Dickson: "Transformation of the antifouling compound irgarol 1051 by Phanerochete crysorium" Water Research. 31(9). 2363-2369 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 青山 勲: "生態影響評価のためのバイオアッセイ" 日本水環境学会セミナー-バイオアッセイと環境化学物質の安全性評価. 80-91 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Aoyama, Isao: "Assessment of cyanazine pwesistence in water" Environmental Toxicology and Water Quality. 11(1). 27-36 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okamura, Hideo: "Ecotoxicity Assessment of Aquatic Environment around Lake Kojima,Japan" Environmental Toxicology and Water Quality. 11(3). 213-221 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 青山 勲: "環境保全工学" 技法堂出版, 227 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 青山 勲: "環境毒性削減:評価と制御 生物多様性のための地球環境技術" 環境技術研究協会, 321 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Isao Aoyama: "Monitoring of Toxic Chemicals Using Daphnia magna Mobility Analysis" Environmental Conservation Engineering. 26-4. 255-259 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isao Aoyama: "Chemical Materials and Ecosystem" Chemistry and Industry. 60-4. 549-551 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dickson Liu: "Transformation of Antifouring Comounds Irgarol 1051 by Phanerochete crysorium" Water Research. 31-9. 2363-2369 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isao Aoyama: "Assessment of Cyanazine Persistence in Water" Environmental Toxicology and Water Quality. 11-1. 27-36 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideo Okamura: "Ecotoxicity Assessment of Aquatic Environment around Lake Kojima, Japan" Environmental Toxicology and Water Quality. 11-3. 213-221 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isao Aoyama: Environemtnal Conservation and Engineering. Gihoudou Publishing Company, 116-130 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isao Aoyama: Toxocity Reduction. Kankyogijutu kenkyuu kyokai, 1-28 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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