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1996 Fiscal Year Final Research Report Summary

Studies on Pi homeostasis by artificial manipulation of intracellular Pi levels.

Research Project

Project/Area Number 07640856
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理
Research InstitutionHITOTSUBASHI UNIVERSITY

Principal Investigator

MIMURA Tetsuro  HITOTSUBASHI UNIVERSITY FACULTY OF COMMERCE,ASSOCIATE PROFESSOR, 商学部, 助教授 (20174120)

Co-Investigator(Kenkyū-buntansha) NEMOTO Setsuko  HITOTSUBASHI UNIVERSITY FACULTY OF SOCIAL SCIENCE,RESEARCH ASSISTANT, 社会学部, 助手 (10017642)
Project Period (FY) 1995 – 1996
KeywordsBarlcy / Cell membrane / Characeae / Inorganic phosphate / Ion homeostasis / Phosphate transport / Plant nutrition / Vacuole
Research Abstract

Inorganic phosphate is one of the essential molecules for plant growth. In natural environment, however, Pi level in soil is extremely low. Even under Pi deficiency, it is well-known that the cytoplasmic Pi level was kept at constant by changing Pi transport activities across the membrane, although the vacuolar Pi level changed related to Pi supply. We call it is Pi homeostasis in plants. However, we have no information, yet how the plant cell detects changes in Pi supply and controls Pi status in the cell. Until now, most of works on the cellular Pi transport mechanisms related to Pi supply were carried out using growing plants or proliferating cultured cells as experimental materials. In these situation, since the cellular physiological status is always changing, it is difficult to analyze Pi homeostatic mechanisms only. In the present study, we tried to control Pi situation independent of cell growth.
First we measured the Pi distribution of barley plants under different Pi situation in order to know the usual experimental conditions. Then we used an isolated and matured Chara internodal cells as material. They do not grow, divide and of course differentiate. Using such cells, we have found first that the removal of Pi from the vacuole by perfusion could not induce any changes in Pi transport activity of the tonoplast. Furthermore, we found that the Pi uptake activity of the plasma membrane also changes independent of both the cytoplasmic and the vacuolar Pi pools.
Besides above, we found that the acid treatment of suspension cultured Catharanthus cells induced changes in the Pi metabolisms. We are now analyzing the relationship of all these phenomena.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Okihara K.: "Furosemide : a specific inhibition of Pi transport across the plasma membrane of plant cells." Plant & Cell Physiology. 36. 53-58 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakano K.: "Lack of control in inorganic phosphate uptake by Catharanthus roseus (L.) G.Don cells." Plant Physiology. 108. 295-302 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mimura T.: "Homeostasis and transport of inorganic phosphate in plants." Plant & Cell Physiology. 36. 1-7 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mimura T.: "Physiological characteristic and regulaion mechanisms of the H^+ pumps in the plasma membrane and tonoplast of Characean cells." Journal of Plant Research. 108. 249-256 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mimura T.: "Studies on distribution,re-translocation and homeostasis of inorganic phosphate in barley leaves." Plant Cell & Environment. 19. 311-320 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Beilby M.J.: "Perfusion of charophyte cells : A critical analysis of the method." J.Experimental Botany. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okihara K., Mimura T., Kiyota S., Sakano K.: "Furosemide : a specific inhibition of Pi trasnport across the plasma membrane of plant cells." Plant & Cell Physiology. 36. 53-58 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakano K., Yazaki Y., Okihara K., Mimura T., Kiyota S.: "Lack of control in inorganic phosphate uptake by Catharanthus roseus (L.) G.Don cells." Plant Physiology. 108. 295-302 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mimura T.: "Homeostasis and transport of inorganic phosphate in plants." Plant & Cell Physiology. 36. 1-7 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mimura T.: "Physiological characteristic and regulaion mechanisms of the H^+ pumps in the plasma membrane and tonoplast of Characean cells." Journal of Plant Research. 108. 249-256 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mimura T., Sakano K., Shimmen T.: "Studies on distribution, re-translocation and homeostasis of inorganic phosphate in barley leaves." Plant Cell & Environment. 19. 311-320 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Beilby M.J., Mimura T., Shimmen T.: "Perfusion of charophyte cells : A critical analysis of the method." J.Experimental Botany. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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