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1996 Fiscal Year Final Research Report Summary

HPLC ANALYSIS OF NEUROTRANSMITIERS IN THE CARDIAC NERVOUS SYSTEM OF CRUSTACEANS

Research Project

Project/Area Number 07640905
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionTOKYO METROPOLITAN UNIVERSITY

Principal Investigator

YAZAWA Tohru  Faculty of Science TOKYO METROPOLITAN UNIVERSITY,Assistant, 理学部, 助手 (30106603)

Co-Investigator(Kenkyū-buntansha) KUWASAWA Kiyoaki  Faculty of Science TOKYO METROPOLITAN UNIVERSITY,Professor, 理学部, 教授 (10015589)
Project Period (FY) 1995 – 1996
Keywordsglutamate / acetylcholine / crustaceans / heart / HPLC / neurotransmitter / isopod / decapod
Research Abstract

It is known that crustaceans have the neurogenic heart whose myocardial cells contract under tight control of rhythmically active neurons in the cardiac ganglion. We have previously demonstrated that myocardium of Bathynomus doederleini was sensitive to glutamate. In pharmacological tests, glutamate, quisqualate and kainate developed tension of myocardial cells in a dose-dependent manner. The dose-response relationship showed an EC50 of about 10-4 M quisqualate, about 3*10-4 M glutamate, and about 5*10-4 M kainate. The order of potency was thus quisqualate>glutamate>kainate. We analyzed glutamate in the extracts of the cardiac ganglion and myocardium of the Bathynomus by means of a technique, high performance liquid chromatography (HPLC). The analysis revealed, respectively, 8741.2+/-184.2pmol/mg and 678.2+/-10.7 pmol/mg for glutamate contents. The present study may show that motor neurons in the cardiac ganglion are glutamatergic.
It has long been repeated that acetylcholine accelerates the heart of decapod crustaceans. Yazawa and Kuwasawa (1992) recently suggested that acetylcholine is the primary candidate for a neurotransmitter of small neurons in the cardiac ganglion of the hermit crab, Aniculus aniculus. We performed a HPLC analysis (with electrochemical detection) of the cardiac ganglion of A.aniculus and Homarus americanus, and detected acetylcholine in the cardiac ganglia of both the animals. We analyzed acetylcholine content in the cardiac ganglia of H.americanus. It was 129+-3.2 fmol/mg wet weight. The present study support the previous postulation (Yazawa and Kuwasawa, 1992) . We conclude that the small neurons in the cardiac ganglion of decapod crustaceans may be cholinergic nerurons, since other neurons and neuronal processes in the ganglion are revealed to be dopaminergic and GABAergic (Yazawa and Kuwasawa, 1992,1994).

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] T.YAZAWA: "An HPLC analysis of glutamate in the ganglion of the isopod crustacean,Bathynomus doederleini." Bulletin of Hosei University at Tama. 12. 1-10 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] J.L.Wilkens: "Evolutionary derivation of the American lobster cardiovascular system:an hypothesis based on morphological and physiological evidence." Invertebrate Biology. 116・1. 30-38 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢沢徹: "神経原性の甲殻類心臓における神経制御と液性制御" Jpn.J.Electrocardiology. 16・suppl 1. S-1-71-S-1-75 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 桑沢清明: "単一細胞レベルでの神経生物学-甲殻類をモデルとした心拍と血流制御の神経機構-" 遺伝. 別刷8号. 70-79 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢沢徹: "甲殻十脚類心臓神経節におけるアセチルコリンのHPLC分析" 法政大学多摩研究報告. 11. 31-38 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢沢徹: "甲殻十脚類心臓神経系におけるドーパミンのHPLC分析" 法政大学多摩研究報告. 10. 1-7 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tohru YAZAWA,Mikinobu YASUMATSU,Minoru OTOKAWA,Kosuke TANAKA,and Kiyoaki KUWASAWA: "An HPLC analysis of glutamate in the ganglion of the isopod crustacean, Bathynomus doederleini." Bulletin of Hosei University at Tama. 12. 1-10 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.L.Wilkens, T.Yazawa, and M.J.Cavey: "Evolutionary derivation of the American lobster cardiovascular system : an hypothesis based on morphological and physiological evidence." Invertebrate Biology. 116. 30-38 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yazawa: "Neural and humoral control of the neruogenic crustacean hearts." Jpn. J.Electrocardiology. vol 16 suppl 1. s-1-71-s-1-75 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kuwasawa and T.Yazawa: "Neurobiology of a single cell level" lden, suppl. 8. 70-79 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yazawa, M.Yasumatsu, M.Otokawa and K.Kuwaswa: "An HPLC analysis of acetylcholine in the cardiac ganglion of decapod crustaceans." Bulletin of Hosei University at Tama. 11. 31-38 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Yazawa, M.Yasumatsu, M.Otokawa and K.Kuwaswa: "An HPLC analysis of dopamine in the cardiac nervous system of decapod crustaceans." Bulletin of Hosei University at Tama. 10. 1-7 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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