Molecular based analyzes of distantly related interspecific and intergeneric hybrids (including somatic hybrids) for useful gene cloning

1996 Fiscal Year Final Research Report Summary

• Project/Area Number: 07660004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: YAMAGATA UNIVERSITY
• Principal Investigator: IMANISHI Shigeru Yamagata University, Faculty of Agriculture, Professor, 農学部, 教授 (40007084)
• Co-Investigator(Kenkyū-buntansha): EGASHIRA Hiroaki Yamagata University, Faculty of Agriculture, Research assistant, 農学部, 助手 (60223632)
• Project Period (FY): 1995 – 1996
• Keywords: Lycopersicon esculentum / Lycopersicon peruvianum / Lycopersicon chilense / Solanum lycopersicoides / interspecific hybrid / somatic hybrid / molecular marker / adventitious shoot formation ability

Research Abstract

Genetic polymorphisms between tomato cultivar Lycopersicon esculentum and the remote wild species should be employed for the molecular genetic analysis of useful traits. The present study reports : (1) Efficiency of ovule selection culture method for the hybridization between tomato and 'peruvianum-complex' along with the development of plant materials for this study : (2) Identification of acid invertase gene for sucrose accumulation in backcross generations B_2, B_3 and B_4 from the cross of tomato and L.peruvianum var.humifusum by using PCR gene diagnosis : (3) Skewed segregation of molecular markers (RAPD,invertase gene and acid phosphatase gene) in backcross generations B_1, and B_2 : (4) Two closely linked RAPD markers and invertase gene located on chromosome 3 to high adventicious shoot formation gene which harbors in L.chilense PI128644, were discovered from the analysis of B_1 and B_2 : (5) Analysis of chloroplast DNA and nuclear genome of somatic hybrid and progenies between tomato and Solanum lycopersicoides by RFLP and GISH.

Research Products

• [Publications] IMANISHI SHIGERU: "Development of interspecific hybrids between Lycopersicon esculentum and L.peruvianum var.humifusum and introgression of L.peruvianum invertase gene" Breeding Science. 46. 356-359 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] TAKASHINA TADASHI: "Evaluation of the cross-incompatibility of 'peruvianum-complex' lines with Lycopersicon esculentum Mill.by the ovule selection method" Breeding Science. 47. 33-37 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] IMANISHI SHIGERU: "Development of interspecific hybrids between Lycopersicon esculetum and L.peruvianum var.humifusum and introgression of L.peruvianum invertase gene into L.esculentum" Breeding Science. 46. 356-359 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] TAKASHINA TADASHI: "Evaluation of the cross-incompatibility of 'peruvianum-complex' lines with Lycopersicon esculentum Mill.by the ovule selection method" Breeding Science. 47. 33-37 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] TAKASHINA TADASHI: "Segregation of high adventitious shoot formation ability and wild species-specific RAPD marker in BC_2F_1 progeny from interspecific hybrid between tomato and a wild species L.chilense" Breeding Science. 47(Supple.1). (1997)
  • Description: 「研究成果報告書概要(欧文)」より