1997 Fiscal Year Final Research Report Summary
Establishment of Monitoring System for Soil-borne Plant Pathogens by Gene Marking
| Project/Area Number |
07660065
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | Kinki University |
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Principal Investigator |
TOYODA Hideyoshi Kinki University, Agriculture, Associate Professor, 農学部, 助教授 (00150805)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Fusarium oxysporum / Gene marking by transformation / in situ hybridization / electroporation / Green fluorescent protein gene / hygromycin-resistant gene / beta-glucuronidase gene / luciferase gene |
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| Research Abstract |
In this reseasrch project for these three years, some soil-borne plant pathogens were successfully transformed with specific marker genes and their infection behavior in soil (before infection) and in planta (after infection into host plants) was monitored by detecting expression of the introduced marker genes. In order to evaluate feasibility or effectiveness of the present method, the author adopted different formae speciales of Fusarium oxysporum whic were indistinguishable one another by the conventional cultural method, and actually attempted to trace and distinguish each fungus. In this study, four formae speciales of F.oxysporum (F.oxy. f. sp. lycopersici, F.oxy. f. sp. fragarea, F.oxy. f. sp. melonis and F.oxy. f. sp. spinaciae) were separately transformed with four different marker genes, hygromycin-resistant gene, beta-glucuronidase gene, luciferase gene and green fluorescent protein gene. These genes were introduced into microconidia of these fungi by electroporation and the gene expression detected using an in situ hybridization. In the actual approach of the present system, host plants were transplanted in soil into which the gene marked pathogens had been introduced and the infected pathogens were detected from both soil and plant tissues as definate intervals after planting. The present method enabled us to simultaneously distinguish different formae speciales of the Fusarium pathogen by detecting different fluorescences emitted from the fluorochrome-labelled probes used for specific hybridization of each marker gene. Thus, the author succeeded in establishing the gene-marking method for monitoring filamentous soil-borne phytopathogenic fungi in the present project research.
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Research Products
(16 results)
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[Publications] Ikeda, S., Toyoda, H., Matsuda, M., Kurokawa, M., Tamai, T., Y., Yoshida, K., Kami, C., Ikemoto, T., Enomoto, M., Shiraishi, K., T., Miyamoto, S., Hanaoka, M., and Ouchi, S.: "Cloning of a chitnase gene chiSHI cloned from gram negative bacterium Kurthia zopfii and control of powdery mildew of barley." Ann. Phytopath. Soc. Japan. 62. 11-16 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Dohgo, H., Toyoda, H., Matsuda, Y., Matsuda, K., Kokuryo, Y., and Ouchi, S.: "Screening of chemical compounds for selectively inhibiting haustorial formation of powdery mildew pathogen in barley coleoptile epidermis." Ann. Phytopath. Soc. Japan. 62. 153-155 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nonomura, T., Toyoda, H., Tanpo, H., Matsuda, Y., Matsuda, K., Gafur, A., Dogo, M., and Ouchi, S.: "Spherical swelling of germ tube and hyphae of Fusarium oxysporum by the treatment with 5-fluoroindole." Ann. Phytopath. Soc. Japan. 62. 414-417 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Fukamizo, T., Honda, Y., Toyoda, H., Ouchi, S., and Goto, S.: "Chitinous component of the cell wall of Fusarium oxysporum, its structure dueduced from chitosanase digestion." Biosci. Biotech. Biochem.60. 1705-1708 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Toyoda, H., Kita, H., kakutani, K., Matuda, Y., Dogo, M., Kato, Y., Nomura, T.Bingo, M., tanpo, H., Chatani, K., shimizu, K,and Ouchi, S.: "Evaluation of stable resistance expression in self-pollinated progenies of bacterial wilt registant regenerants obtained from leaf callus of tomato." Plant Biotechnol.14. 105-110 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Toyoda, H., Matsuda, Y., Dogo, M., Tanpo, H., Sekimoto, K., and Ouchi, S.: "Gene expression in tomato for glucosylation of indole-3-propionic acid, a selective antibacterial agent against a bacterial wilt pathogen, Pseudomonas solanacearum." Plant Biotechnol.14. 111-116 (1997)
Description
「研究成果報告書概要(欧文)」より
