1996 Fiscal Year Final Research Report Summary
Genome rearrangements in Bradyrhizobium japonicum field isolates.
| Project/Area Number |
07660077
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | TOHOKU UNIVERSITY (1996)
Ibaraki University (1995) |
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Principal Investigator |
MINAMISAWA Kiwamu INSTITUTE OF GENETIC ECOLOGY,TOHOKU UNIV.ENVIRONMENTAL INFORMATION PROFESSOR, 遺伝生態研究センター, 教授 (70167667)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Bradyrhizobium japonicum / insertion sequence / genome arrangement / gene transfer / soybean / transposon |
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| Research Abstract |
Among Bradyrhizobium japonicum field isolates, I found abnormal isolates (HRS isolates) which possesses many copies of prokaryotic insertion sequences (IS), RS-alpha, RS-beta and others. We obtained physical evidence for genome rearrangements in B.japonicum HRS isolates. The symbiotic regions (nif, nod and hup) in a HRS genome were heavily subjected to insertion, deletion and other types of genome rearrangements. HRS isolates had a tandem repeat RS-alpha dimer, which may give rise to a burst of transpositional rearrangements.
Because snapback analysis showed the presence of the pairs of ISs in an opposite direction, these structures correspond to type II transposon, a compound transposon. In order to examine whether the potential compound transposon may contribute to horizontal transfer of symbiotic genes, I developed to detect nod gene transfer on agar media from B.japonicum HRS isolates to B.elkanii USDA94deltaNOD,a mutant defective in nod genes. As a result, we could obtained B.elkanii cells which acquired the nod region of the HRS isolate and IS element, FK1. The B.elkanii cells restored nodulation phenotype to siratro and production of lipo-chitin nodulation signals.
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Research Products
(12 results)
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[Publications] Yuhashi, K., Akao, S., Fukuhara, H., Tateno, E.Chun, J., Stacey, G., Hara, H., Kubota, M., Asami, T.and Minamisawa, K.: "Bradyrhizobium elkanii induces outer cortical root swelling in soybean." Plant Cell Physiol.36. 1571-1577 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Minamisawa, K., Nakatsuka, Y., Seki, T.and Isawa, T.: Soybean bradyrhizobia population dynamics : The presence of highly reiterated RS-alpha possessing field isolates in Japan. In Nitrogen Fixation : Fundamentals and Applications. Edited by I.A.Tikhonovich, N.A.Provorov, V.I.Romanov, and W.E.Newton.Kluwer Academic, 711 (1995)
Description
「研究成果報告書概要(欧文)」より
