1996 Fiscal Year Final Research Report Summary
STRUCTURE OF ACTIVE SITE REGIONS ON EXO-GLYCOSIDASES
| Project/Area Number |
07660087
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
MATSUI Hirokazu Hokkaido Univ., Fac.of Agriculture, Associate professor, 農学部, 助教授 (90109504)
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| Project Period (FY) |
1995 – 1996
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| Keywords | alpha-glucosidase / dextranase / exo-type / Active site / cDNA / Primary structure |
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| Research Abstract |
Sugar beet alpha-glucosidase was modified with CBE.An analysis of an CBE-labeled peptide isolated from the digest of modified enzyme showed that the sequence around the essential amino acid was -DGIWIDMNE-. A cDNA encoding sugar beet alpha-glucosidase was cloned from a library constructed from mRNA of suspension cultured cells. The cDNA had an open reading frame encoding a polypeptide of 913 amino acid residues. The deduced amno acid sequence indicated relatively high homology in the range of 28.2 to 54.3% to those for other alpha-glucosidases.
The gene encoding isomaltotrio-dextranase was cloned from Brevibacterium fuscum, and analyzed. The deduced amno acid sequence (641 residues) had 80% of homology to that of Arthrobacter dextranase.
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