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1997 Fiscal Year Final Research Report Summary

Functional analysis of eubacterial small cytoplasmic RNA (scRNA)

Research Project

Project/Area Number 07660091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionUniversity of Tsukuba

Principal Investigator

NAKAMURA Kouji  University of Tsukuba, Institute of Biological Sciences Assistant professor, 生物科学系, 講師 (40212097)

Project Period (FY) 1995 – 1997
KeywordsSRP RNA / small cytoplasmic RNA / scRNA / cubacteria / protein translation / translation / signal recognition particle / SRP
Research Abstract

B.subtilis scRNA is a member of SRP RNA family. By depleting scRNA in vivo, we have obtained a direct evidence that scRNA is essential for viability, protein secretion and translation. It is transcribed as a 354-nt primary transcript and processed at both 5' and 3' sites. In precursor, 5' and 3' flanking sequence encompassing the processing sites is complement. Therefore, it is predicted that both processing sites located within a double stranded stem structure. This substrate specificity is identical to that of well konwn ribonuclease III (RNaseIII). To examine the function of Bs-RNaseIII in processing of pre-scRNA,in vitro ^<32>P-labeled pre-scRNA (15ng) was incubated with the purified Bs-RNaseIII (1.5ng). Results indicate that pre-scRNA is primarily cleaved by Bs-RNaseIII at both 5' and 3' sites.
Based on human SRP RNA,all most eukaryotic SRP RNA can be divided into four functional domains (domains I to IV). In addition to domain III,domains I and II are absent in most eubacteria except B.subtilis. The SRP RNA (scRNA) of B.subtilis consists of domains I,II and IV.Functional analysis demonstrated that deletion of domains I and/or II blocked formation of heat-resistant spore. Moreover, SRP RNA of another spore-forming bacteria, clostridium perfringens, contains three domains, and can restore the growth and sporulation of B.subtilis conditional mutant, suggesting that both domains I and II are required for sporulation. However, more extended phylogenetic studies revealed that non-spore forming gram-positive bacteria, such as staphylococcus aureus, Streptococcus pyogenesis, Corynebacterium glutamicum and Listeria monocytogenesis, also contain domains I and II like B.subtilis. The domains I and II are conserved throughout the evolution of gram-positive bacteria. This fact indicates that other important function will be included in domains I and II.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Shibata, T.: "Depletion of small cytoplasmic R-NA(scRNA)confers fusidic-acid resistanceon Bacillus subtilis." Biochem.Biophys.Res.Commun.210. 317-323 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oguro, A.: "srb.a Bacillus subtilis gene enc-oding a homologue of the α-subunit of the mammalian signal recognition par-ticle receptor." DNA Res.2. 95-100 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, K.: "Small cytoplasmic RNA(scRNA)gene from Clostridiumperfringens can replace the gene for the Bacillus subtilis scRNAin both growth and sporulation." Microbiology. 141. 2965-2975 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Oguro, A.: "The effect of Srb,a homologue of mammalian SRP receptor α-subunit,on Bacillus subtilis growth and protein tr-anslocation." Gene. 172. 17-24 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shibata, T.: "Identification of protein synthesis elongation factor G(EF-G)as a 4.5S R-NA-binding protein in Escherichia coli." J.of Biol.Chem.271. 13162-13168 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurita, K.: "Identification of a region of Ba-cillus subtilis Fifh,a homologue of mam-malian SRP54 protein,that is essential for binding to small cytoplasmic RNA(sc-RNA)。" J.of Biol.Chem.271. 13140-13146 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bunai, k.: "Bacillus subtilis Ffh,a homologueof mammalian SRP54,can intrinsically b-ind to the precursors of secretory prot-eins." Biochem.Biophys.Res.Commun.227. 762-767 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takamatsu, H.: "Identification of a region requi-red for binding to presecretory protein in Bacillus subtilis Ffh,a homologue of the 54 kilodalton subunit of mammalian singal recognition particle." Eur.J.Biochem.248. 575-582 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村 幸治: "枯草菌における蛋白質分泌系と分泌装置" 日本農芸化学会誌. 69. 43-46 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山根 國雄: "細菌性シグナル認識粒子SRP構造・機能の哺乳類SRPとの比較" 蛋白質・核酸・酵素. 41. 1569-1579 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村 幸治: "真正細菌SRP RNAの多機能性" 日本生化学会誌. 69. 192-196 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shibata, T., Y.Nakamura, K.Nakamura and K.Yamane: "Depletion of small cytoplasmic RNA (scRNA) confers fusidic-acid resistance on Bacillus subtilis" Biochem.Biophys.Res.Commun. 210. 317-323 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oguro, A., H.Kakeshita, K.Honda, H.Takamatsu, K.Nakamura, and K.Yamane: "srb : a Bacillus subtilis gene encoding a homologue of the alpha-subunit of the mammalian signal recognition particle receptor" DNA Res. 2. 95-100 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, K., E.Hashizume, T.Shibata, Y.Nakamura, S.Mala and Y.Yamane: "Small cytoplasmic RNA (scRNA) gene from Clostridium perfringens can replace the gene for the Bacillus subtilis scRNA in both growth and sporulation" Microbiology. 141. 2965-2975 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oguro, A., H.Kakeshita, H.Takamatsu, K.Nakamura, and K.Yamane: "The effect of Srb, a homologur of mammalian SRP receptor alpha-subunit, on Bacillus subtilis growth and protein translocation" Gene. 172. 17-24 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shibata, T., Y.Fujii, Y.Nakamura, K.Nakamura, and K.Yamane: "Identification of protein synthesis elongation factor G(EF-G)as a 4.5S RNA-binding protein in Escherichia coli" J.of Biol. chem. 271. 13162-13168 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurita, K., K.Honda, S.Suzuma, H.Takamatsu, K.Nakamura, and K.Yamane: "Identification of a region of Bacillus subtilis Fth, a homologue of mammalian SRP54 protein, that is essential for binding to small cytoplasmic RNA(scRNA)" J.of Biol. Chem. 271. 13140-13146 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Bunai K.H.Takamatsu, T.Horinaka, A.Oguro, K.Nakamura, K.Yamane: "Bacillus subtilis Fth, a homologue of mammalian SRP54, can intrinsically bind to the precursors of secretory proteins" Biochem. Biophys. Res. Commun. 227. 762-767 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takamatsu, H., K.Bunai, T.Horinaka, A.Oguro, K.Nakamura, K.Yamane: "Identification of a region required for binding to presecretory protein in Bacillus subtilis Fth, a homologue of the 54 kilodaton subunit of mammalian signal recognition particle" Eur. J.Biochem. 248. 575-582 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, K and K.Yamane: "Protein translocation machinery in Bacillus subtilis (in japanese)" Nippon Nogeikagaku kaishi. vol.69. 43-46 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamane, K., H.Takamatsu and K.Nakamura: "Comparisons with mammalian SRP on their structure and function (in Japanese)" Protein, Nucleic Acid and enzyme. vol.41. 1569-1579 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, K and K.Yamane: "Identification of eubacterial SRP RNA as a multifunctional molecule" Seikagaku. vol.69. 192-196 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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