1996 Fiscal Year Final Research Report Summary
Development and Characterization of Novel Derivatives expected from the Structure and Function of Dialdehyde Cellulose
| Project/Area Number |
07660213
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産学
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| Research Institution | SHIZUOKA UNIVERSITY |
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Principal Investigator |
MAEKAWA Eiichi Faculty of Agriculture, Professor, Shizuoka University, 農学部, 教授 (00027161)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Nobuyuki Faculty of Agriculture, Professor, Shizuoka University, 農学部, 教授 (70023439)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Dialdehyde cellulose / Periodate oxidation / Polycarboxylic derivative / Polyhydric alcohol derivative / Characterization by NMR / Oxime cellulose / Diamino cellulose / Immobilized enzyme complex |
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| Research Abstract |
This research project is first concerned with characterization of polycarboxylic and polyhydric alcohol derivatives by NMR Spectroscopy, which are obtained from oxidation and reduction of diadehyde cellulose derived by periodate oxidation of cellulose. Secondly, this research project includes preparation and characterization of novel amino group-containing derivatve (amino cellulose), which is followed by reduction of oximation products obtained by reaction of dialdehyde cellulose with hydroxylamine. Novel immobilized enzyme complex was prepared by covalent condination of amino cellulose and enzyme (alpha-amylase) with glutaldehyde, and the characteristic properties of the product were examined. Thus, the following results were concluded : 1) It has been proved that detailed analyzes of signal assigned to methyl and carbonyl groups in acetyl group in ^1H and ^<13>C NMR spectra of polyalcohol peracetate give evidence distinguishable by identification of the corresponding signals for periodate oxidized and unoxidized glucose residues. 2) The effect in NMR spectra by oxidation-spitting at the C-2 and C-3 of glucose resides in the cellulose molecule was analyzed from shift behavior of resonance signals. 3) Electromicroscopic observation of immobilized enzyme complex prepared above by SEM gave a spheric like mass between fiber segments. 4) By coating treatment of the immobilized enzyme complex with sodium alginate, great enhancement of retention by repeated usage and heat stability was shown.
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