1996 Fiscal Year Final Research Report Summary
Regulation mechanisms in granulosa cell apoptosis in follicular atresia
| Project/Area Number |
07660379
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
MANABE Noboru Kyoto University, Department of Animal Science, Associate Professor, 農学部, 助教授 (80243070)
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| Project Period (FY) |
1995 – 1996
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| Keywords | apoptosis / granulosa cells / cumulus cells / oocytes / ovarian follicular atresia / proteases / monoclonal antibodies / cell membrane glycoprotein-receptors |
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| Research Abstract |
The porcine antral follicles were dissected from the ovaries of mature pigs, and then granulosa and cumulus cells were isolated from each follicle. In atretic follicles, high activity of neutral Ca2+/Mg2+-dependent endonuclease and DNA ladder formation estimated by electrophoresis were noted only in granulosa cells but not in cumulus cells. Extremely low activity of the endonuclease and no DNA ladder formation were observed in both types of cells obtained from healthy follicles. Moreover, apoptotic cells were observed histochemically among granulosa cells only. A good correlation between the endonuclease activity of granulosa cells and the progesterone/estradiol ratio of follicular fluid in each follicle was found. These results suggest that apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pigs.
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[Publications] Noboru Manabe, Tetsuro Suzuki, Nobuyuki Kuribayashi, Makoto Miyamoto, Keiko Kuramitsu, Makoto Kojiro, Yoshiharu Matuura, Tatsuo Miyamura, Seigo Takamatsu, Nobuo Nagano, and Hajime Miyamoto.: "Liver fibrosis is highly correlated with hepatocellular apoptosis and transforming growth factor-beta1 mrna expression in chronic hepatitis B." Hepatology. 24. 366-367 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Noboru Manabe, Yasuyoshi Azuma, Yukinobu Kuribayashi, Makoto Miyamoto, Keiko Kuramitsu, Yoshihiro Furuya, Nobuo Nagano, and Hajime Miyamoto.: The 8th Animal Science Congress : Immunohistochemical quantification of fast-myosin in frozen histological sections of the goatlimb muscles.The Japan Society of Zootechnical Sciences, Tokyo, Japan, 620 (1996)
Description
「研究成果報告書概要(欧文)」より
