1996 Fiscal Year Final Research Report Summary
ROLE OF MUCOSAL MAST CELLS IN THE EXPULSION OF SEVERAL SPECIES OF STRONGYLOIDES FROM GASTROINTESTINAL TRACTS
| Project/Area Number |
07660405
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Miyazaki University |
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Principal Investigator |
HORII Yoichiro MIYAZAKI UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (80173623)
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| Co-Investigator(Kenkyū-buntansha) |
MAKIMURA Susumu MIYAZAKI UNIVERSITY,FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (80003127)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Strongyloides / mast cell / gastrointestinal tract / mucosa / protective mechanism / goblet cell / chymase / typtase |
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| Research Abstract |
Adult worms of canine Strongyloides (Strongyloides stercoralis), bovine Strongyloides (S.papillosus) and porcine Strongyloides (S.ransomi) were intraduodenally inoculated into W/W^V and +/+ mice. The expulsion of S.stercoralis from W/W^V mice was significantly slower than that from +/+ mice. While S.papillosus and S.ransomi were expelled immediately and there were no differences between W/W^V mice and +/+ mice. When S.papillosus adult worms were intraduodenally transferred into other rodent than mice, the worms highly established in hamsters and Mongolian gerbils but not in rats. However, after establishment of S.papillosus adult worms, it caused paralytic ileus and host death in the gerbils. Detailed mechanisms of this paralytic ileus are now under investigation.
We succeeded cDNA clonings of a tryptase and 2 chymases form mast cells of the intestinal mucosae of Mongolian gerbils. When the sequences and the expression in the tissues of these proteases were compared to those of rats and mice, the proteases from gerbil mast cells were similar to those of connective tissue mast cells of rats and mice. RNA blotting revealed that the mast cell proteases of gerbils were not identical between skin mast cells and mucosal mast cells.
There is a possibility that the expulsive mechanism (s) of S.papillosus and S.ransomi is different from that of S.ratti, S.venezuelensis and S.stercoralis.
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[Publications] MURAKUMO,Y., IDE,H., ITOH,H., TOMITA,M., KIBAYASHI,T., MARUYAMA,H., HORII,Y.& NAWA,Y.: "Cloning of the cDNA encoding mast cell tryptase of Mongolian gerbil, Meriones unguiculatus, and its preferential expression in the intestinal mucosa." Biochemical Journal. 309. 921-926 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] TIURIA,R., HORII,Y., MAKIMURA,S., ISHIKAWA,N., TSUCHIYA,K.& NAWA,Y.: "Effect of testosterone on the mucosal defense against intestinal helminths in Indian soft-furred rats, Millardia meltada with reference to goblet and mast cell responses." Parasite Immunology. 17. 479-484 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] ITOH,H., MURAKUMO,Y., TOMITA,M., IDE,H., KOBAYASHI,T., MARUYAMA,H., HORII,Y.& NAWA,Y.: "Cloning of the cDNAs for mast-cell chymases from the jejunum of Mongolian gerbils, Meriones unguiculatus, and their sequence similarities with chymases expressed in the connective-tissue mast cells of mice and rats." Biochemical Journal. 314. 923-929 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] TATEISHI,M., HORII,Y., MARUYAMA,H., NAWA,Y., TSUCHIYA,K.& MAKIMURA,S.: "Sensitive enzyme-linked immunosorbent assay (ELISA) to measure parasite-specific antibodies of Indian soft-furred rats, Millardia meltada." Journal of Veterinary Medical Science. (in press).
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「研究成果報告書概要(欧文)」より
