1996 Fiscal Year Final Research Report Summary
Identification of receptor-binding site of the mouse hepatitis virus spike protein
| Project/Area Number |
07660413
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | National Institute of Neuroscience, NCNP |
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Principal Investigator |
TAGUCHI Fumihiro National Institute of Neuroscience, NCNP,section chief, 神経研究所・モデル動物開発部, 室長 (30107429)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Hideka National Institute of Neuroscience, NCNP,postdoc
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| Project Period (FY) |
1995 – 1996
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| Keywords | mouse hepatitis virus / coronavirus / spike protein / receptor-binding site |
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| Research Abstract |
We have previously shown that the N terminal region composed of 330 amino acids of MHV S protein (S1N330) is important for receptor-binding activity, since the S1N330 protein expressed by recombinant vaccina virus binds to the MHV-receptor protein prepared on the membrane paper by Western blot in a similar manner as whole S protein binds to the receptor protein. The aim of this experimetn is to identify the amino acids that participate in the receptor-binding activity in S1N330. We have found that 7MHV strains can use mmCGM1 as a functional receptor. In the S1N330 of these 7 MHV strains, we found 3 major consercative regions which include more than 10 consective amiano acids identical among these 7 MHV strains. We have introduced the mutations in some of amino asids in these regions and analyzed the receptor-binding activity of such mutant proteins. The mutant S1N330 protein with a mutation at amino acid 62 from the N terminus failed to bind to the receptor protein. The receptor binding og a mutant with mutations at 212,214, and 216 was remarkably reduced. These results imply that amino acids at 62,212,214 and 216 play important role for the receptor-binding activity of the MHV s protein. Furthermore, it is suggested that secondary or tertiary structure of SIN330 is necessary for receptor-binding activity, since the region containing amino acid 62 is located apart from a region including amino acids 212,214 and 216 in primary MHV s protein sturucture.
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