1996 Fiscal Year Final Research Report Summary
Cytochemical analysis on the mechanisms of biosynthesis and action of steroid hormones.
| Project/Area Number |
07670019
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | The University of Tokushima, School of Medicine |
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Principal Investigator |
ISHIMURA Kazunori The university of Tokushima, School of Medicine, Department of Anatomy, Professor, 医学部, 教授 (90112185)
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| Project Period (FY) |
1995 – 1996
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| Keywords | 5 alpha-Reductase / Immunocytochemistry / Electron microscope / Steroid hormones / Anterior pituitary / Prostate / Adrenal gland / Schwann cells |
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| Research Abstract |
(1) Immunocytochemical localization of 5 alpha-reductase was studied in various tissues using polyclonal antibodies against type 1 and 2 isozymes.
(2) In the rat anterior pituitary, type 1 isozyme was present in the membrane of rough endoplasmic reticulum of gonadotrophs.
(3) In the rat adrenal cortex, zonae fasciculata-reticularis cells contain 5 alpha-reductase type 1. The expression of the enzyme increased in the castrated animals. This indicates that sex steroids play a role in the regulation of corticoids secretion and metabolism.
(4) In the rat adrenal medulla, 5 alpha-reductase type 1 was present in the supporting cells surrounding chromaffin cells and Schwann cells around nerve fivers.
(5) Steroid 5 alpha-reductase was present in the satellite cells in the ganglions in addition to Schwann cells.
(6) In the human prostate, seminal vesicle and vas deferens, type 2 isozyme was present in the basal epithelial cells and smooth muscle cells in the interstitial tissue.
(7) Leucocyte type arachidonate 12-lipoxygenase was immunolocalized in the secretory cells of pig anterior pituitary gland and neutrophils of dog.
(8) Platelet type arachidonate 12-lipoxygenase was immunocytochemically localized in the plateletes and eosinophils of the peripheral blood, megakaryocytes of the bone marrow, and keratinocytes of the epidermis.
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