1995 Fiscal Year Final Research Report Summary
PATHOLOGICAL AND MOLECULAR BIOLOGICAL RESEARCH ON BIOLOGICAL FEATURES OF PEDIATRIC TUMORS
| Project/Area Number |
07670210
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Yokohama City University |
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Principal Investigator |
MISUGI Kazuaki YOKOHAMA CITY UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF PATHOLOGY, PROFESSOR, 医学部・病理学第二, 教授 (50106332)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Ichiro YOKOHAMA CITY UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF PATHOLOGY, ASSISTANT PROFESSOR, 医学部・病理学第二, 助教授 (00184028)
MIYAGI Yohei YOKOHAMA CITY UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF PATHOLOGY, ASSISTANT, 医学部・病理学第二, 助手 (00254194)
NAGASHIMA Yoji YOKOHAMA CITY UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF PATHOLOGY, LECTURER, 医学部・病理学第二, 講師 (10217995)
FUNABIKI Tetsunori YOKOHAMA CITY UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF PEDIATRICS, ASSISTANT, 医学部・小児科学, 助手 (20264616)
SASAKI Yoshiro KANAGAWA CHILDRENS MEDICAL CENTER DIVISION OF PATHOLOGY, DIRECTOR, 病理, 部長
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| Project Period (FY) |
1995 – 1996
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| Keywords | Pediatric tumor / Spontaneous regression / Apoptosis / Cell death / bcl-2 / TRKA / TS-TG / LNGFR |
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| Research Abstract |
Pediatric tumors are characterized by occasional spontaneous regression, as well as their rapid and aggressive growth. We examined this unique feature from the point of view of apoptotic cell death, especially on mass screened neuroblastomas (MS-NBs), whioh have favorable prognoses and even occasionally regress spontaneously. Histological examination of MS-NBs revealed individual cell death and apoptotic bodies, but extracted genomic DNA failed to showtypical ladder-like cleavage. Further, we performed northern blot analysis to assess the expression of high affinity nerve growth factor receptor (TRKA), low affinity nerve growth receptor (LNGFR) and tissue transglutaminase (TS-TG), which are known to be intimately related to apoptosis. MS-NBs were classified into two groups: 1) which show high expression of TRKA and 2) which show high expression of LNGFR and TS-TG. The former seemed to differentiate to nerve cell lineage, whereas the latter cause apoptotic cell death. (Miyagi et al., submitted)
We also histopathologically examined pediatric tumors with tendency of spontaneous regression, but failed to obtain evidense of apoptosis.
At present, we are planning to evaluate the effects on cell growth, differentiation and cell death caused by transfection of TRKA, LNGFR and TS-TG genes.
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