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1996 Fiscal Year Final Research Report Summary

Host exchange of Lyme disease borreliae during tick feeding

Research Project

Project/Area Number 07670269
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionAsahikawa Medical College

Principal Investigator

NAKAO Minoru  Asahikawa Medical College, Department of Parasitology, Assistant, 医学部, 助手 (70155670)

Project Period (FY) 1995 – 1996
KeywordsIxodes persulcatus / Lyme disease / borreliae / transmission
Research Abstract

Lyme disease is a newly recognized systemic infection caused by spirochetes of the genus Borrelia. In this study, the host exchange of borreliae during tick feeding process was examined histologically by using Borrelia garinii, Ixodes persulcatus ticks, and mice. In infected murine skin, the number of borreliae was extremely low ; however, they seemed to be wrapped around collagen fibers in dermis. The number of borreliae in the murine skin was estimated at<1000 per 10 mg by the nested PCR analysis using flagellin gene-based primers. During tick feeding process, borreliae in murine skin were sucked into larval ticks and transstadially transmitted to nymphal ticks through the molting process. In unfed infected nymphs, borreliae were localized in their midgut lumen. The dynamics of borrelial migration in feeding ticks was unclear ; however, borreliae gradually increased in the salivary glands, supporting a hypothesis of salivary transmission of borreliae. The number of borreliae in salivary glands of 48 hours-fed nymphs was estimated at<10 cells per tick by the fluorescent antibody technique. This small number of borreliae was sufficient to induce the murine infection. The mechanism of midgut penetration and salivary invasion of borreliae in ticks still remains to be elucidated. In the future, the transformation of borreliae from the silent form to the infective form during the feeding process of ticks should be examined.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Sato Y and Nakao M: "Transmission of the Lyme disease spirochete, Borrelia garinii, between infected and uninfected immature Ixodes persulcatus during cofeeding on mice." Journal of Parasitology. 83(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Konishi T and Nakao M: "Antibiotic treatment in mice infected with Japanese Borrelia garinii : efficacy of ceftriaxone for eradicating the infection induced by Ixodes persulcatus tick bites." Microbiology and Immunology. 41. 165-168 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukunaga M, Okada K, Nakao M, Konishi T, and Sato Y: "Phylogenetic analysis of Borrelia species based on flagellin gene sequences and its application for molecular typing of Lyme disease borreliate." International Journal of Systematic Bacteriology. 46. 898-905 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakao M, Uchikawa K, and Dewa H: "Distribution of Borrelia species associated with Lyme disease in the subalpine forests of Nagano prefecture, Japan" Microbiology and Immunology. 40. 307-311 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakao M and Sato Y: "Refeeding activity of immature ticks of Ixodes persulcatus and transmission of Lyme disease spirochete by partially fed larvae." Jouranal of Parasitology. 82. 669-672 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato Y and Nakao M.: "Transmission of the Lyme disease spirochete, Borrelia garinii, between infected and uninfected immature Ixodes persulcatus during cofeeding on mice." Journal of Parasitology. 83, (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Konishi T and Nakao M.: "Antibiotic treatment in mice infected with Japanese Borrelia garinii : efficacy of ceftriaxone for eradicating the infection induced by Ixodes persulcatus tick bites." Microbiology and Immunology. 41 (2). 165-168 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukunaga M,Okada K,Nakao M,Konishi T,and Sato Y.: "Phylogenetic analysis of Borrelia species based on flagellin gene sequences and its application for molecular typing of Lyme disease borreliae." International Journal of Systematic Bacteriology. 46 (4). 898-905 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakao M,Uchikawa K,and Dewa H.: "Distribution of Borrelia species associated with Lyme disease in the subalpine forests of Nagano prefecture, Japan." Microbiology and Immunology. 40 (4). 307-311 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakao M and Sato Y.: "Refeeding activity of immature ticks of Ixodes persulcatus and transmission of Lyme disease spirochete by partially fed larvae." Journal of Parasitology. 82(4). 669-672 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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