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1996 Fiscal Year Final Research Report Summary

Basis research on pathogenetic mechanisms of Toxoplasmic retinochoroiditis.

Research Project

Project/Area Number 07670283
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionChiba University (1996)
Nagasaki University (1995)

Principal Investigator

YANO Akihiko  Chiba Univ.Sch.Med., Dept of Parasitology, Professor, 医学部, 教授 (20135122)

Co-Investigator(Kenkyū-buntansha) AOSAI Fumie  Nagasaki Univ.Sch.Med., Dept of Med.Zoology, Lecturer, 医学部, 講師 (80150316)
Project Period (FY) 1995 – 1996
KeywordsToxoplasmic retinochoroiditis / CD4^+CTL / Toxoplasma gondii / Melanoma / gamma-IFN / Uveitis / SAG1 gene / Quantitative competitive PCR
Research Abstract

The most severe clinical symptoms of congenital toxoplasmosis are retinochoroiditis, encephalomyelitis and hydrocephalus, skin rush, together with hepatosplenomegaly and lymphadenopathy. Some of these organs and tissues contain melanocytes. Vogt-Koyanagi-Harada disease ahs believed as an autoimmune disease in which melanocytes are target cells of autoimmune responses in the patients. To examine the possibility of the mechanisms operative in toxoplasmic retinochoroiditis and Vogt-Koyanagi-Harada disese, we analyzed the cytotoxic responses of PBL of toxoplasmosis patients which were induced by Toxoplasma gindii (T.gondii) -infected melanoma cells and of PBL of Vogt-Koyanagi-Harada disease induced by melanoma cells. T.gondii-infected human melanoma cell-specific CD4+CTL were induced from the PBL of patients with toxoplasmosis by stimulation with T.gondii-infected melanoma cells. Similarly, melanoma-specific CD4+CTL cells were induced from PBL of Vogt-Koyanagi-Harada disease by the stimulation HLA-DR matched melanoma cells. These CD4+CTL cells produced a large amount of IFN-gamma and IL-6 in response to the stimulation by T.gondii-infected melanoma cells in toxoplasmosis patients or melanoma cells in Vogt-Koyanagi-Harada disease. HLA-DR expression of T.gondii-infected melanoma cells was upregulated by both IFN-gamma and IL-6, but antigen presentation of T.gondii-infected melanoma cells to T.gondii-infected cell-specific CD4+CTL was upregulated by only IFN-gamma but not by IL-6. Thus, human melanocytes infected by T.gondii may play an important role in immunopathogenic as in toxoplasmic chorioretinitis through the production of these lymphokines. Similarly, melanocytes may be immunopathogenic in Vogt-Koyanagi-Harada diseas.

  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Yang,T-H.,Aosai,F.,Norose,K.,Ueda,M.and Yano,A.: "Enhanced cytotoxicity of IFN-γ-producing CD4^+ cytotoxic Tlymphocytes specific for T.gondii-infected human melanoma cells." J.Immunol.150. 290-298 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wang,X-C.,Norose,K.,Yano,A.,Ohta,K.and Segawa,K.: "Two-color flow cytometric analysis of activated T lymphocytes in aqueous humor of patients with endogenous vs. exogenous uveitis." Curr.Eye Res.14. 425-433 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sano,K.and Yano,A.: "Differential γ δT cell response controlled by antigen presenting cells in Toxoplasma gondii-infected mice." Jpn.J.Parasitol.44. 19-27 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Luo,W.,Seki,T.,Yamashita,K.,Aosai,F.,Ueda,M.and Yano,A.: "Establishment of quantitative detection method of Toxoplasma gondii by quantitative competitive polymerase chain reaction of surface specific antigen-1(SAG1)gene." Jpn.J.Parasitol.44. 183-190 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chikuba,N.,Akazawa,S.,Yamaguchi,Y.,Kawasaki,E.,Takino,H.,Takao,Y.,Maeda,Y.,Okuno,S.,Yamamoto,H.,Yokota,A.,Yoshimoto,M.,Ohe,N.,Yamashita,K.,Yano,A,and Nagataki,S.: "Type 1(insulin-dependent)diabetes mellitus with Graves' disease is not associated with DR9 antigen but associated with combination of DQA1^*0301 and DPB1^*0501 in Japanese." Diabetes Res.Clin.Pract.27. 31-37 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Aosai,F.,Yang,T-H.,Norose,K.,He,N.,Ueda,M.and Yano,A.: "Comparative study of interferon-γ production by Toxoplasma gondii-infected cell-specific CD8^+ CTL and CD4^+ CTL." Jpn.J.Parasitol.44. 210-217 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ye,Q.H.,Kobayashi,S.,Fujiwara,T.,Nozawa,S.Ohta,H.,Kiguchi,K.,Tojo,R.,Kobayashi,J.,Sumi,K.,Yano,A.and Takeuchi,T.: "Occurence of metronidazole-resistant Trichomonas vaginalis in Japan." Jpn.J.Parasitol.44. 473-480 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohta,K.,Norose,K.,Wang,X-C.,Ito,S.,Yano,A. and Segawa,K.: "Apoptosis-related fas antigen on memory T lymphocytes in aqueous humor from patients with uveitis." Curr.Eye Res.15. 299-306 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Norose,K.,Tokushima,T.and Yano,A.: "Quantitative polymerase chain reactien in diagnosing ocular toxoplasmosis." Am.J.Ophthalmol.121(4). 441-442 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yang,T-H.,Aosai,F.,Norose,K.,Ueda,M.and Yano,A.: "Differential regulation of HLA-DR expression and antigen presentation in Toxoplasma gondii-infected melanoma cells by interleukin 6 and interferon γ." Microbiol.Immunol.40(6). 443-449 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamashita,K.,Ueda,M.and Yano,A.: "CD8^+ T-cell mediated protection against acute toxoplasmosis in mice induced with X-ray irradiated Toxoplasma tachyzoites." Jpn.J.Parasitol.45. 173-180 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Norose,K.and Yano,A.: "Melanoma-specific Th1 cytotoxic T-lymphocyte lines in Vogt-Koyanagi-Harada disease." British Journal Ophtalmology.80(10). 1002-1008 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Luo,W.and Yano,A.: "Kinetics of changes in abundance of transcripts encoding cytokines,Fas and Fas ligand in susceptible and resistant mice infected with Toxoplasma gondii." Trop.Med.38(1). 27-37 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamakawa,R.,Yamashita,Y.,Yano,A.,Morita,J.and Kato,H.: "Congenital toxoplasmosis complicated with central diabetes insipidus in a Down syndrome infant(patient report)." Brain Dev.18. 75-77 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Luo W.,Aosai F.,Ueda M.,Yamashita K.,Shimizu K.,Sekiya S.and Yano A.: "Kinetics of changes in parasite load in susceptible and resistant mice infected with an avirulent strain of Toxoplasma godii by using quantitative competitive PCR." J.Parasitol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 神薗慎太郎,山下裕史朗,矢野明彦,上田耕一郎,西見寿博,加藤裕久: "先天性トキソプラスマ症の1例-PCR法による迅速診断の有用性-" 日本新生児学会雑誌. 31. 533-537 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 糸数直哉、此元隆雄、井上忍、園田徹、杉本徹、矢野明彦: "先天性トキソプラズマ症患児治療後の髄液中に検出されたトキソプラズマ特異的DNA-puantitative competitive PCR法による評価の意義-" 脳と発達. 28(3). 264-266 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢野明彦: "抗原提示細胞.アレルギーメディカル用語ライブラリー、実験医学別冊 羅智靖,大田健,河野陽一,古江増隆 編" 羊土社,東京., 20-23 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢野明彦: "生化学辞典(第3版)(3項目分担)" 東京生化学同人,東京.(印刷中),

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 矢野明彦: "抗原提示能 IX.リンパ球・APCの機能のin vitro検査" 臨床免疫(印刷中),

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yang, T-H., Aosai, F., Norose, K., Ueda, M.and Yano, A: "Enhanced cytotoxicity of IFN-gamma-producing CD4^+ cytotoxic Tlymphocytes specific for T.gondii-infected human melanoma cells." J.Immunol.150. 290-298 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wang, X-C., Norose, K., Yano, A., Ohta, K.and Segawa, K.: "Two-color flow cytometric analysis of activated T lymphocytes in aqueous humor of patients with endogenous vs.exogenous uveitis." Curr.Eye Res.14. 425-433 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sano, K.and Yano, A: "Differential gamma delta T cell response controlled by antigen presenting cells in Toxoplasma gondii-infected mice." Jpn.J.Parasitol.44. 19-27 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Luo, W., Seki, T., Yamashita, K., Aosai, F., Ueda, M.and Yano, A.: "Establishment of quantitative detection method of Toxoplasma gondii by quantitative competitive polymerase chain reaction of surface specific antigen-1 (SAG1) gene." Jpn.J.Parasitol.44. 183-190 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chikuba, N., Akazawa, S., Yamaguchi, Y., Kawasaki, E., Takino, H., Takao, Y., Maeda, Y., Okuno, S., Yamamoto, H., Yokota, A., Yoshimoto, M., Ohe, N., Yamashita, K., Yano, A and Nagataki, S.: "Type 1 (insulin-dependent) diabetes mellitus with Graves' disease is not associated with CR9 antigen but associated with combination of DQA1^<**>0301 and DPB1^<**>0501 in Japanese." Diabetes Res.Clin.Pract.27. 31-37 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Aosai, F., Yang, TH-H., Norose, K., He, N., Ueda, M.and Yano, A.: "Comparative study of interferon-gamma production by Toxoplasma gondii-infected cell-specific CD8^+CTL and CD4^+CTL." Jpn.J.Parasitol.44. 210-217 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ye, Q.H., Kobayashi, S., Fujiwara, T., Nozawa, S., Ohta, H., Kiguchi, K., Tojo, R., Kobayashi, J., sumi, K., Yano.A and Takeuchi, T.: "Occurence of metronidazole-resistant Trichomonas vaginalis in Japan." Jpn.J.Parasitol.44. 473-480 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohta, K., Norose, K., Wang, X-C., Ito, S., Yano, A.and Segawa, K.: "Apoptosis-related fas antigen on memory T lymphocytes in aqueous humor from patients with uveitis." Curr.Eye Res.15. 299-306 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Norose, K., Tokushima, T.and Yano, A.: "Quantitative polymerase chain reaction in diagnosing ocular toxoplasmosis." Am.J.Ophthalmol.12 (4). 441-442 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yang, T-H., Aosai, F., Norose, K., Ueda, M.and Yano, A.: "Differential regulation of HLA-DR expression and antigen presentation in Toxoplasma gondii-infected melanoma cells by interleukin 6 and interferon gamma" Microbiol.Immunol.40 (6). 443-449 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamashita, K., Ueda, M.and Yano, A.: "CD8^+ T-cell mediated protection against acute toxoplasmosis in mice induced with X-ray irradiated Toxoplasma tachyzoites." Jpn.J.Parasitol.45. 173-180 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Norose, K.and Yano, A.: "Melanoma-specific Th1 cytotoxic T-lymphocyte lines in Vogt-Koyanagi-Harada disease." British Journal Ophtalmology.80 (10). 1002-1008 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Luo, W.and Yano, A.: "Kinetics of changes in abundance of transcripts encoding cytokines, Fas and Fas ligand in susceptible and resistant mice infected with Toxoplasma gondii." Trop.Med.38 (1). 27-37 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamakawa, R., Yamashita, Y., Yano, A., Morita, J.and Kato, H.: "Congenital toxoplasmosis complicated with central diabetes insipidus in a Down syndrome infant (patient report)." Brain Dev.18. 75-77 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Luo W., Aosai F., Ueda M., Yamashita k., Shimizu K., Sekiya S.and Yano A.: "Kinetics of changes in parasite load in susceptible and resistant mice infected with an avirulent strain of Toxoplasma godii by using quantitative competitive PCR." J.Parasitol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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