1996 Fiscal Year Final Research Report Summary
Characterization of stage specific antigen of Schistosoma sp.recognized by monoclonal antibodies
| Project/Area Number |
07670289
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Dokkyo University School of Medicine |
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Principal Investigator |
MATSUDA Hajime Dokkyo Univ.Sch.Med., Professor, 医学部, 教授 (30114648)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAI Satoru Dokkyo Univ.Sch.Med., Research Associate, 医学部, 助手 (70275733)
KIRINOKI Masashi Dokkyo Univ.Sch.Med., Research Associate, 医学部, 助手 (50265302)
CHIGUSA Yuichi Dokkyo Univ.Sch.Med., Assoc.Prof., 医学部, 助教授 (20171936)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Schistosoma / Schistosoma japonicum / Monoclonal antibody / Specific antigen / species specificity |
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| Research Abstract |
A monoclonal antibody (MoAb), termed 1-7B,which recognized a gut-associated excretory-secretory antigen, was produced by using spleen cells of Schistosoma japonicum infected mouse. We characterized the antigen on a two-dimensional gel electrophoretic pattern (2-D pattern) of proteins extracted from S.Japonicum adult worms. The antigen formed one vertically elongated spot, with an apparent molecular weight of around 100 kDa and an isoelectric point of less than 4. The spot was evident on a 2-D pattern of S.japonicum-infected rabbit serum, indicating that it was a circulating antigen. In addition, because of its lectin binding activity and V8 protease sensitivity, we termed the antigen circulating glyco-proteinaceous antigen (CGPA).
Next, a polyclonal rabbit antiserum against CGPA was prepared and used for detection of CGPA on 2-D patterns and paraffin sections of adult S.japonicum and S.mansoni worms. The results showed that CGPA distributed on cytoplasm of gut epithelial cells, only in S.japonicum. Since all five CAs of adult S.japonicum, reported previously by other authours, were known to have immunologically related antigens in adult S.mansoni, CGPA characterized by MoAb 1-7B have not been reported before, on the grounds of differences in their specificity and molecular weight from those in previous reports.
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Research Products
(12 results)
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[Publications] Yokoi, K., Kirinoki, M., Chigusa, Y., Yamamoto, H., Ishii, T., Imai, S.and Matsuda, H.: "Studies on antibody responses in mice to egg antigens of Schistosoma japonicum and S.mansoni detected by circumoval precipitin (COP) reaction and enzyme-linked immunosorbent assay (ELISA)." Jpn.J.Parasitol. 45 (4). 299-308 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yasuraoka, K., Blas, B.L., Matsuda, H., Irie, Y., Nihei, N., Ohmae, H., Yokoi, H., Hambre, R., Pangilinan, R., Autentico, C.and Tanaka, H.: "Approaches to the elimination of schistosomiasis on Bohol Island, Philippines." Jpn.J.Parasitol.45 (5). 391-399 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yokoi, K., Kirinoki, M., Chigusa, Y., Ishii, T., Imai, S.and Matsuda, H.: "Sequential changes in IgM and IgG subclass antibody responses in mice to egg antigens of Schistosoma japonicum revealed by enzyme-linked immunosorbent assay (ELISA) and immunoblotting." Jpn.J.Parasitol.45 (5). 400-407 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Chigusa, Y., Kirinoki, M., Yokoi, H., Kawai, S., Matsuda, H., Yasuraoka, K., Bugawan, L.E.and Blas, B.L.: "A survey of intestinal parasitic infections in San Narciso, Victoria, Oriental Mindoro, Philippines." Jpn.J.Trop.Med.Hyg.25 (1). 25-27 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yokoi, H., Kirinoki, M., Chigusa, Y., Ishii, T., Imai, S.and Matsuda, H.: "Characterization of Schistosoma japonicum specific egg antigen recognized by circumoval precipitin (COP) producing monoclonal antibodies." (submitted to Parasit.Internat.).
Description
「研究成果報告書概要(欧文)」より
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