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1996 Fiscal Year Final Research Report Summary

Regulatory Mechanism for Expression of Genes for Iron Uptake in Pseudomonas aeruginosa

Research Project

Project/Area Number 07670314
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionOkayama University

Principal Investigator

TSUDA Masataka  Okayama University, Faculty of Science, Associate Professor, 理学部, 助教授 (90172022)

Co-Investigator(Kenkyū-buntansha) NAKAZAWA Teruko  Yamaguchi University School of Medicine, Professor, 医学部, 教授 (40053053)
Project Period (FY) 1995 – 1996
KeywordsPseudomonas aeruginosa / Iron Uptake / Siderophore / Pyoverdin / Pathogenic Factors / Transcriptional regulation / Site-specific resolution / Deletion mutants
Research Abstract

Under iron limitation, Pseudomonas aeruginosa secretes siderophore (pyoverdin) to chelate and uptake the ferric iron. Most of the pyoverdin biosynthetic (pvd) genes are located in a chromosomal 103-kb region, termed as pvd region, and this region also encodes FpvA,an outer membrane protein that serves as a receptor for ferri-pyoverdin complex, and PvdS,an activator required for transcription of the pvd operons. Transcription of these operons is further repressed under iron-rich conditions by Fur, a global repressor of the iron regulon. Sequence analysis and Fur titration assay demonstrated that the Fur box, a consensus sequence for binding of Fur, is absent in the three pvd promoter regions but present at the promoter region of pvdS.Transcription of pvdS was repressed and depressed under iron-rich and -limiting conditions, respectively. These results strongly support that no transcription of the pvd operons under iron-rich conditions is due to the Fur-mediated transcriptional repression of the pvdS gene. The pvdS mutant produced alkaline proteinase one-fourth in amount of that the wild type strain produced. This indicates that PvdS plays an important, albeit not essential, role in production of alkaline proteinase. Various mutants having large and defined deletion in the chromosomal pvd region were isolated using a site-specific resolution system of Tn1722. Such a mutant completely lacking the pvd region grew normally in minimal media, demonstrating that the pvd region does not carry any essential or auxotrophic genes. The deletion mutanats were constructed that retained and lacked the fpvA gene. The former mutanat, but not the latter one, had the ability to uptake the externally added ferri-pyoverdin complex, implying that a second low-affinity receptor other than FpvA appears not to exist in P.aeruginosa.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Hiroaki Miyazaki: "A positive regulatory gene,pvdS,for expression of pyoverdin biosynthetic genes in Pseudonanas aeruginosa" Molecular and General Genetics. 248. 17-24 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 津田雅孝: "シュードモナス属細菌のトランスポゾンの解析とトランスポゾンを用いた分子遺伝学的解析" 日本細菌学雑誌. 50. 947-959 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 津田雅孝: "緑膿菌の鉄獲得系遺伝子群の解析" 緑膿菌感染症研究会講演記録. 29. 65-69 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuko Abe: "A genetic analysis system of Burkholderia cepacila : construction of mobilizable transposons and a cloning vector" Gene. 174. 191-194 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomoko Mizote: "The thiJ locus and its relation ship to phosphorylation of hydroxy methyl pyrimidire in Escherichia coli" Microbiology. 142. 2969-2974 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masataka Tsuda: "Molocular Biology of Pseudomonads" ASM Press, 10 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 津田雅孝: "遺伝生態情報の可能性" 東北大学遺伝生態研究センター, 16 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyazaki, H.: "A positive regulatory gene, pvdS,for expression of pyoverdin biosynthetic genes in Pseudomonas aeruginosa PAO" Molecular and General Genetics. 248. 17-24 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuda, M.: "Study on Pseudomonas transposons and molecular genetic analysis of Pseudomonas by use of transposons (in Japanese)" Japanese Journal of Bacteriology. 50. 947-959 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuda, M.: "Analysis of genes involved in pyoverdin production in Pseudomonas aeruginosa PAO (in Japanese)" Proceedings of Study Group of Pseudomonas aeruginosa Infection. 29. 65-69 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Abe, M.: "A genetic system of Burkholderia cepacia : construction of mobilizable transposons and a cloning vector" Gene. 174. 191-194 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mizote, T.: "The thiJ locus and its relation to phosphorylation of hydroxymethylpyrimidine in Escherichia coli" Microbiology (UK). 142. 2969-2974 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuda, M.: Molecular Biology of Pseudomonads. ASM Press, 10 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuda, M.: Current Topics in Genetic Ecology (in Japanese). Institute of Genetic Ecology, Tohoku University, 16 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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