1996 Fiscal Year Final Research Report Summary
Influenza virus transmission and the change of viral binding specificity toward the molecular species of sialic acid
| Project/Area Number |
07670349
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | University of Shizuoka |
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Principal Investigator |
SUZUKI Takashi University of Shizuoka, School of Pharmaceutical Sciens, Associate Professor, 薬学部, 助教授 (20240947)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Influenza virus / receptor / sialic acid |
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| Research Abstract |
To elucidate the relation between the receptor recognition of influenza A viruses and the molecular species of sialic acid in the host trachea, we determined the ratio of Neu5Gc to Neu5Ac in human and animals respiratory epitheliums by fluorometric high-performance liquid chromatography, and examined the binding specificity of swine influenza virus strains for gangliosides containing different molecular species of sialic acid by a virus overlay assay on a silica gel plate. The presence of N-glycolylneuraminic acid (Neu5Gc), which had not been entirely detected in human tracheal epitheliums, and N-acetylneuraminic acid (Neu5Ac) in swine tracheal epitheliums was observed in a 1 : 1 ratio. A remarkable difference was observed in the ratio of Neu5Ac of to Neu5Gc between human and animals tracheal epitheliums. Four of the swine influenza virus H 1 and H 3 isolates tested, recognized II^3Neu5GcLacCer containing Neu5Gc as well as II^3Neu5AcLacCer. we have found that equine influenza viruses preferentially bound to the sialyloligosaccharides containing Neu5Gc. In addition, the ratio of Neu5Gc to Neu5Ac in equine respiratory epitheliums was higher than that of swine. These results suggest that swine influenza viruses recoginze sialylsugar chains containing the molecular species of sialic acid present predominantly in the swine tracheal epithelium.
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