1996 Fiscal Year Final Research Report Summary
IgHintron enhancer acttivity and somatic hypermutation
| Project/Area Number |
07670377
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Immunology
|
|---|
| Research Institution | Science University of Tokyo |
|---|
Principal Investigator |
AZUMA Takachika Science University of Tokyo, Research Institute for Biological Science, Professor, 生命科学研究所, 教授 (00028234)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Keywords | somatic hypermutation / intron enhancer / immunoglobulin genes / transgenic mice |
|---|
| Research Abstract |
Chloramphenicol acetyltransferase (CAT) transgenic mice were used for study the variation of intron enhancer (Emu) activity during the late stage of B cell development. Science the expression of CAT transgene was regulated by VH promoter and Emu, a change in the CAT activity provides a measure of transcriptional activity of Ig genes. When the resting B cells were stimulated by cross-linking with goat anti-IgM antibodies, the CAT activity decreased approximately 1/10. On the other hand, 20-30% increase in the CAT activity was observed by stimulation with lipopolysacharide (LPS). The stimulation of B cells with LPS caused an increase in the CD43^+B cells that possesed a higher CAT activity than CD43-B cells. On the other hand, CD43^+PNA^+B cells obtained by stimulation with antigens in vivo also showed increased CAT activity, suggesting the enhanced transcriptional activity of Emu. These results suggest that Emu activity was augmented in B cwlls where somatic mutation is induced with high frequency.
|
