1996 Fiscal Year Final Research Report Summary
Exploration of the DNA-recognition site for anti-DNA antibody inducing DNA-binding protein nucleobindin
| Project/Area Number |
07670528
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KUBOTA Tetsuo Faculty of Medicine, Tokyo Medical and Dental University, associate professor, 医学部, 助教授 (90205138)
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| Project Period (FY) |
1995 – 1996
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| Keywords | nucleobindin / anti-DNA antibody / DNA-binding protein / autoimmunity |
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| Research Abstract |
We have achieved the following progresses in the studies on physiological activity and fine characteristics of nucleobindin.
1. Administration of recombinant nucleobindin induced production of autoantibodies such as anti-DNA antibody even in normal BALB/c mice that have no genetical background of autoimmune disorders.
2. Enhanced expression of nucleobindin mRNA was observed in the lymphoid organs of lupus-prone strains of mice such as MRL/lpr, C3H/gld, and male BXSB.
3. Enhanced synthesis of nucleobindin in lupus-prone mice was demonstrated also in the protein level by immunohistochemical staining using an antibody produced against neclebindin peptide.
4. A monoclonal antibody to human nucleobindin successfully immunoprecipitated native nucleobindin in the nuclear extract of human malignant lymphoma cell line Molt-4.
5. The oligonucleotides which showed high binding affinity to human nucleobindin were selected from synthesized oligonucleotides containig 26-base-long random sequences. Some motif were repeatedly found in the selected oligonucleotides. Furthermore, such motif as CCACCACAATA and GTGTGGA were found in the up-stream region of IL-2 gene. It remains elusive how nucleobindin is involved in regulation of gene transcription.
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