• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1996 Fiscal Year Final Research Report Summary

Exploration of the DNA-recognition site for anti-DNA antibody inducing DNA-binding protein nucleobindin

Research Project

Project/Area Number 07670528
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KUBOTA Tetsuo  Faculty of Medicine, Tokyo Medical and Dental University, associate professor, 医学部, 助教授 (90205138)

Project Period (FY) 1995 – 1996
Keywordsnucleobindin / anti-DNA antibody / DNA-binding protein / autoimmunity
Research Abstract

We have achieved the following progresses in the studies on physiological activity and fine characteristics of nucleobindin.
1. Administration of recombinant nucleobindin induced production of autoantibodies such as anti-DNA antibody even in normal BALB/c mice that have no genetical background of autoimmune disorders.
2. Enhanced expression of nucleobindin mRNA was observed in the lymphoid organs of lupus-prone strains of mice such as MRL/lpr, C3H/gld, and male BXSB.
3. Enhanced synthesis of nucleobindin in lupus-prone mice was demonstrated also in the protein level by immunohistochemical staining using an antibody produced against neclebindin peptide.
4. A monoclonal antibody to human nucleobindin successfully immunoprecipitated native nucleobindin in the nuclear extract of human malignant lymphoma cell line Molt-4.
5. The oligonucleotides which showed high binding affinity to human nucleobindin were selected from synthesized oligonucleotides containig 26-base-long random sequences. Some motif were repeatedly found in the selected oligonucleotides. Furthermore, such motif as CCACCACAATA and GTGTGGA were found in the up-stream region of IL-2 gene. It remains elusive how nucleobindin is involved in regulation of gene transcription.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Kanai,Y.: "Induction of autoantibodies in normal mice by injection of nucleobindin and natural occurrence of antibodies against nucleobindin in autoimmune MRL/lpr/lpr mice." Immunology Letters. 45. 35-42 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iizuka,H.: "Enhanced expression of nucleobindin in lymphatic organs of lupus-prone mice." Lupus. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kanai Y,Takeda O,Miura K,Amagai M,Kaneko T,Kubota T,Kanai Y,Tanuma S,Kurosawa Y: "Induction of autoantibodies in normal mice by injection of nucleobindin and natural occurrence of antibodies against nucleobindin in autoimmune MRL/lpr/lpr mice." Immunol Lett. 45. 35-42 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iizuka H,Kubota T,Satake F,Hirokawa G,Miura K,Kurosawa Y,Miyasaka N,Kanai Y: "Enhanced expression of nucleobindin in lymphatic organs of lupus-prone mice." Lupus. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi