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1996 Fiscal Year Final Research Report Summary

Study on signal transduction pathway via integrins in autoimmune T cells

Research Project

Project/Area Number 07670539
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionSaitama Medical School

Principal Investigator

TAKEUCHI Tsutomu  Dep of Medicine, Saitama Medical School, Associate Professor, 医学部, 助教授 (50179610)

Co-Investigator(Kenkyū-buntansha) MORI Sigehisa  Dep of Medicine, Saitama Medical School, Assistant Professor, 医学部, 講師 (10190993)
Project Period (FY) 1995 – 1996
KeywordsTyrosine phosphorylation / Integrins / Signal transduction / pp125FAK
Research Abstract

It is now well recognized that integrins and their ligands are pivotal role in the first step of inflammation such as the adhesion between the leukocytes and the endothelial cells. Here, we showed data by a confocal image analyzer that the double stainig signals by anti-beta1 and anti-phosphotyrosine were observed in the perivascular and sublining areas in RA synovial cells, while it was not detected in OA,raising a possibility that the adhesive interaction not only mediates adhesion, but delivers the signals into the cell, contributing to maintain inflammation in RA synovium. To explore the mechanism, we examined the tyrosine phosphorylation of synovial cells in vitro culture system. The immunoblot for hospho-tyrosine demonstrated the five major proteins form the synovial cells in RA,while few faint bands were detected in OA.Since the pp125, among five major bands, was most prominent in RA synovial cells, we next examined whether pp125 is FAK or not.
Immunoprecipitation with anti-pp125FAK has demonstrated that the band was pp125FAK itself and the tyrosine phosphorylation of pp125FAK was significantly elevated in RA synovial cells. Furthermore, confocal laser microscopy showed that the signals by anti-phosphotyrosine and anti-pp125FAK were abundantly detected in macrophage-like synovial cells.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Takeuchi T.,et al: "Tyrosine phosphorylated protein in synovial cells of RA" Int Rev Immunol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeuchi T.,et al: "Reduced PTP ase activity of CD45 on PBL in patients with SLE" Clin Exp Immunol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeuchi T.,et al: "Characterization of SM17 reacting on activating antigen" Tissve Antigen. 48(4). 356 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koide J,et al.: "Spontaneous establishment of EBV-infected fibroblast from RA" J Virol. 71(3). 2478-81 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsubota K.,et al.: "Biopsy of labial salivary glands in the diagnosis of SjS" J Rheum. 23. 76-82 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeuchi T.,et al: "Characterization of tyrosine phosphorylation in synoriel cells" Arth Rheum. 38. 214 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹内 勤: "免疫疾患-state of arts" 今西二郎,淀井淳司,湊 長博,山本一彦 編, 467 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 竹内 勤: "図説病態内科講座" 高久史磨 編, 300 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeuchi T., et al.: "Tyrosine phosphorylated protein in synovial cells of RA." Int Rev Immunol. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeuchi T., et al.: "Reduced PTPase activity of CD45 on PBL in patients with SLE." Clin Exp Immunol. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeuchi T., et al.: "Characterization of SM17 reacting on activating antigen." Tissue Antigen. 48 (4). 356 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koide J., et al.: "Spontaneous establishment of EBV-infected fibloblast from RA." J Virol. 71 (3). 2478-81 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsubota K., et al.: "Biopsy of labial salivary glands in the diagnosis of SjS." J Rheum. 23 (2). 76-82 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeuchi T., et al.: "Characterizaion of tyrosine phosphorylation in synovial cells." Arth Rheum. 38 (6). 214 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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