1996 Fiscal Year Final Research Report Summary
THERAPY FOR HEPATIC FIBROSIS BY TARGETED DELIVERY OF ANTI-FIBROTIC AGENTS TO HEPATIC STELLATE CELLS
| Project/Area Number |
07670572
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | UNIVERSITY OF TOKYO |
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Principal Investigator |
MASAKI Naohiko UNIV.OF TOKYO,FACULTY OF MEDICINE,INSTRUCTOR, 医学部・附属病院, 助手 (40219316)
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| Co-Investigator(Kenkyū-buntansha) |
TOMIYA Tomoaki UNIV.OF TOKYO,FACULTY OF MEDICINE,INSTRUCTOR, 医学部・附属病院, 助手 (90227637)
IKEDA Hitoshi UNIV.OF TOKYO,FACULTY OF MEDICINE,INSTRUCTOR, 医学部・附属病院, 助手 (80202422)
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| Project Period (FY) |
1995 – 1996
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| Keywords | STELLATE CELL / RETINOL BINDING PROTEIN / COLLAGEN |
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| Research Abstract |
(1) Retinol binding protein (RBP) was purified from rat serum using transthyretin. Iodinated RBP was added to the culture medium of rat stellate cells on the 7th day of primary culture. Cell associated radio activity was not affected in the presence of a 100-fold excess of unlabeled RBP,suggesting that there was no specific binding to the cells. Our observation indicates that the existence of RBP receptor in the stellate cells is controversial. It is still to be clarified whether RBP is useful in the targeted delivery of anti-fibrotic agents to hepatic stellate cells.
(2) Type I procollagen antisense was added to the culture medium of rat stellate cells. Collagen production was unchanged with up to 50 muM antisense. Our results suggest that effective introduction to the cells of antisense should be considered.
(3) Possible anti-fibrotic action of tranilast, an anti-allergic agent, was examined. Tranilast inhibited collagen production by stellate cells at the pre-translational level. Reduced TGFbeta1 gene expression by tarnilast may be one of the mechanism of inhibition of collagen production.
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