1996 Fiscal Year Final Research Report Summary

STUDY OF EXPRESSION IF VEGF AND IT'S RECEPTOR

Research Project

Project/Area Number	07670627
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Gastroenterology
Research Institution	KANAZAWA MEDICAL UNIVERSITY
Principal Investigator	KAWAHARA Hiromu KANAZAWA MEDICAL UNIVERSITY,MEDICINE,ASOCIATED PROFESSOR, 医学部, 講師 (10177727)
Co-Investigator(Kenkyū-buntansha)	SAWADA Makoto KANAZAWA MEDICAL UNIVERSITY,MEDICINE,ASSISTANT, 医学部, 助手 (50170824)
Project Period (FY)	1995 – 1996
Keywords	VEGF / Hepatocellural carcinoma / Necrosis / Metastasis
Research Abstract	Expression of VEGF was studied in human hepatoma derived cell line (HepG2) and human hepatoma tissues. VEGF is known of four subtypes of messenger RNA, due to splicing sites. Three subtypes of VEGF, VEGF_<121>, VEGF_<165> and VEGF_<189>, were expressed, by HepG2 cells using RT-PCR with a set of primers detecting whole length mRNA from exoni to exon8. In non-regenerative nodules, hepatocytes were stained weakly by immunohistochemistry with VEGF antibody. Smooth muscle cells of small vessels and duct epithelial cells in the fibrous stroma were also positive for VEGF.In human hepatoma, hepatoma cells, especially in periphery of necrotic parts, were stained markedly. In addition, hepatoma cells invading the small vessels of fibrous stroma were also strongly positive for VEGF stain. These results indicated that hepatoma cells in vitro and in vivo expressed VEGF supporting neovascularization and metastasis.