1996 Fiscal Year Final Research Report Summary
Role of extracellular matrix, cytokines and gut hormones on pancreatic fibrosis in arginine-induced pancreatitis.
| Project/Area Number |
07670638
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | University of Occupational and Environmental Health |
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Principal Investigator |
OTSUKI Makoto University of Occupational Health and Environmental Health, School of Medicine Professor, 医学部, 教授 (00030916)
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| Co-Investigator(Kenkyū-buntansha) |
KIHARA Yosuyuki University of Occupational Health and Environmental Health, School of Medicine A, 医学部, 助手 (80279330)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Pancreatitis / Fibrosis / Extracellular Matrix / TGF-beta / mRNA |
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| Research Abstract |
Pancreatitis was induced by intraperitoneal injection of L-arginine (500mg/100g body weight) in male Wistar rats weighing 150-160g. Rats were sacrificed at 24,36,48,60h, and 3,5, and 7 days after the injection. Total RNA was extracted from the pancreas using acid-guanidium thiocyanate-phenol-chloroform method. Expressions of extracellular collagen matrix messenger RNA (mRNA) were determined by Northern transfer analysis and that of TGF-beta mRNA was examined by reverse transcriptase polymelase chain reaction (RT-PCR). TGF-beta mRNA expression was reached the peak value at 48h, thereafter, it gradually decreased, but remained elevated until 7 days. Fibronectin mRNA expression was detectable at 24 h after the arginine injection, reaching the peak value at 48 h. Type-I collagen mRNA expression was increased at 48 h and peaked on day 5. An increase of type-III collagen mRNA expression was detected at 48 h, reaching the peak value at 72 h. Furthermore, an expression of type-IV collagen mRNA was delayd ; detectable at 60 h and peaked at 5 day, but lasted until 7 days. Since TGF-beta appeared at the earliest stage of pancreatitis, it is likely that TGF-beta is related to the production of extracellular matrix. TGF-beta and some extracellular matrix might be involved in the fibrosis of pancreatitis.
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