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1996 Fiscal Year Final Research Report Summary

Contribution of apoptosis onto pathogenesis of neuro-Behcet's disease

Research Project

Project/Area Number 07670735
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionFukuoka University

Principal Investigator

KANEOKA Hidetoshi  Fukuoka University Hospital. Assoc. Prof., 病院, 助教授 (20161169)

Project Period (FY) 1995 – 1996
KeywordsBehcet's disease / neuro-Behcet's disease / apoptosis / Fas antigen / neutrophil / HLA-B51
Research Abstract

Based on recent findings from our laboratory as well as from others, we believe that hyperfunction of neutrophils involves in pathogenesis and pathophysiology of Behcet's disease. Neuro-Behcet's disease is a subset of the disease, characterized by typical mucocutaneogenital disorders, involvement in central nervous system, and poor prognosis. In my proposal to this grant was to ask whether this abnormality in neutrophils is brought by apoptosis of neutrophils themselves or of immunocompetent cells including antigen presenting macrophges or immunoregulating Tlymphcytes.
To demonstrate apoptosis, lestablished to detect apopttic fragments by DNA banding on agarose gel electrophoresis, and by flow cytometry originally established by Nicoletti, et al. I also utilised flow cytometer to demonstrate apoptosis related molecules, Fas antigen (CD 95), Fas ligand, and bcl-2, utilizing flow cytemeter and their monoclonal antibodies, on and in those cells, and RT-PCR from mRNA from lymphocytes.
I had very hard time to see phisiological apoptosis in neutrophils quite quickly after cell preparation. I failed to demonstrate any difference of apoptosis of neutrophils between patients and healthy controls by any means which I had choose. Next I turned cells for experiments from neutrophils to T lymphocytes.
DNA banding pattern of lymphocytes from patients were similar to those from controls, however the expression of Fas and Fas ligand was increased significantly in patients group.
Based on those findings, I started to do experiments with cells from patients with neuro-Behcet's disease. I found it very difficult to recruit the patients because of very low incidence of the disease, and to collect fresh cells before physiological apoptosis. Only one case could be determined the apoptosis, and found no difference from other patients without neurological involvements. I need further experiment to establish hypothesis described.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Toshiharu Nakajima,et al: "Induction of differentiation into monocyle/macrophage cell lineage of a human cosinophilic leukemia cell line,EoL-1 by simultaneous stimulation with tumour necrosis factor-α and interferon-γ." British Jounal of Haematology. 89. 258-265 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuhiro Takeno,et al: "Excessive function of peripheral blood neutrophils from patients with Behcet's disease and from HLA-B51 transgenic mice." Arthritis and Rheumatism. 38. 426-433 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 坂根剛,兼岡秀俊: "血液病と自己免疫,1,自己免疫の最近の考え方." 日常診療と血液. 5. 303-313 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川崎俊三ら: "蜂刺症によりネフローゼを発症し、自然寛解を得た一例." 日本内科学会雑誌. 86. 143-145 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naomi Yamashita,et al: "Role of γδ T lymphocytes in the development of Behcet's disease." Clinical and Experimental Immunology. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuhiro Takeno,et al: "Autoreactive T cell clones from patients with SLE support polyclonal autoantibody production." Journal of Immunology. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 兼岡秀俊ら: "人類遺伝学-基礎と応用改訂第2版" 金原出版・東京, 476 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 兼岡秀俊: "臨床検査ガイド 97" 文光堂・東京, 1079 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiharu Nakajima, et al.: "Induction of differentiation into monocyte/macrophage cell lineage of a human eosinophilic leukemia cell line, EoL-1 by simultaneous stimulation with tumour necrosis factor-alpha and interferon-gamma." British Journal of Haematology. 89 (2). 258-265 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuhiro Takeno, et al.: "Excessive function of peripheral blood neutrophils from patients with Behcet's disease and from HLA-B51 trangenic mice." Arthritis and Rheumatism. 38 (3). 426-433 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuyoshi Sakane, Hidetoshi Kaneoka: "Hematological disorders and autoimmunity. (written in Japanese)" Practical Hematology. 5 (3). 303-313 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shunzo Kawasaki, et al.: "A case report with nephrotic syndrome due to bee sting and with spontaneous recovery. (written in Japanese)" Journal of the Japanese Society of Internal Medicine.86 (1). 143-145 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naomi Yamashita, et al.: "Role of gammadelta T lymphocytes in the development of Behcet's disease." Clinical and Experimental Immunology.(in press.). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuhiro Takeno, et al.: "Autoreactive T cell clones from patieents with SLE support polyclonal autoantibody." Journal of Immunology.(in press.). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hidetoshi Kaneoka, et al.: Variable expression of the genes. (written in Japanese). Human Genetics - Principles and Practice, 2nd ed., edit. T.Yanase. p57-68. Kanehara & Co., Tokyo., (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hidetoshi Kaneoka: Lymphoblast formation. (written in Japanese). Clinical Management of Laboratory Data in medical Practice 1997, edit. K.Wada, et al. p745-749. Bankodo, Tokyo., (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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