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1996 Fiscal Year Final Research Report Summary

A ROLE OF CYTOKINE IN PROGRESSION OF HEART FAILRE

Research Project

Project/Area Number 07670750
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionYamagata University School of Medicine

Principal Investigator

YAMAGUCHI Seiji  Yamagata Univ.Sch.of Med., Instructor, 医学部, 講師 (30239892)

Co-Investigator(Kenkyū-buntansha) SHIRAKABE Masanori  Yamagata Univ.Sch.of Med., Instructor, 医学部, 助手 (10241697)
Project Period (FY) 1995 – 1996
KeywordsCONGESTIVE HEART FAILURE / TUMOR NECROSIS FACTOR (TNF) / SOLUBLE TNF RECEPTORS / CYTOKINE
Research Abstract

TNFalpha exerts its functions by binding to two specific receptors (TNF-RI and TNF-RII). The extracellular domains of TNF-RI and-RII are proteolytically cleaved (sTNF-RI and sTNF-RII) and neutralize the effects of TNF alpha. We investigated the surface and gene expressions of TNF-RII and the shedding of TNF-RII on mononulear leukocytes in patients with congestive heart failure (CHF). Mononuclear leukocytes were separated from peripheral blood in 14 control and in 21 patients with CHF.The surface expression of TNF-RII by immunofluorescence flow cytometry was increased in relation to the severity of CHF (NYHA class). TNF-RII mRNA level by Northern blot analysis was relatively higher in patients with advanced CHF.To assess the feasibility of shedding TNF-RII in ^ハmononuclear leukocytes, the cells from patients with CHF and control subjects were incubated in the absence and presence of phorbol myristate acetate (PMA). sTNF-RII in the supernatant with PMA was higher in CHF than control (p<.05). Furthermore, shedding of TNF-RII was higher in the supernatant of mononuclear leukocyts of volunteers treated with 10% serum from CHF than those from control (p<.05). In conclusion, TNF-RII on mononuclear leukocytes was up-regulated in patients with CHF.In addition to feasibility of shedding of TNF-RII on mononuclear leukocytes in CHF,serum from patients with CHF enhanced shedding of TNF-RII on the cells. The presence of CHF affects TNF alpha-TNF receptor system which may modify systemic manifestations including alteration of immune system in CHF.

  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Akio Fukui: "Increasing coronary perfusion pressure on diastolic and systolic performance is less pronounced in right ventricll than left ventricll" Cardiovascular Research. 31. 899-906 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naoki Nozaki: "Soluble tumor necrosis factor receptors are elevated in relation to severity of congestive heart failure" Japanese Circulation Journal. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masaki Okuyama: "Serum levels of soluble form of Fas molecule in patients with congestive heart failure" The American Journal of Cardiology. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukui A,Yamaguchi S,et al: "Increasing coronary perfusion pressure on diastolic and systolic performance is less pronounced in right ventricle than in left ventricle." Cardiovasc Res. 31. 899-906 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomoike H,Yamaguchi S,et al: "Two major pumps of the Heart : Difference and Interdependence of the Right and Left Ventricles. -New Horizons for Failing Heart Syndrome-" S.Sasayama (ed.) Springer-Verlag Tokyou. 79-103 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naoki N,Yamaguchi S,et al: "Resetting of regional preload due to ventricular shape Resetting of regional preload due to ventricular shape change alters diastolic and systolic performance." Jpn Circ J. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okuyama M,Yamaguchi S,et al: "Serum levels of solible form of Fas molecule in patients with congestive heart failure." Am J Cardiol. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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