1995 Fiscal Year Annual Research Report

血管平滑筋のイオンチャネルの機能とその制御機構に関する研究

Research Project

Project/Area Number	07670760
Research Category	Grant-in-Aid for General Scientific Research (C)
Research Institution	The University of Tokyo
Principal Investigator	中島敏明東京大学, 医学部(病), 助手 (50227790)
Co-Investigator(Kenkyū-buntansha)	浜田栄治東京大学, 医学部(病), 医員挾間比左徳東京大学, 医学部(病), 医員
Keywords	Vascular smooth muscle / Ionic currents / Vasopressin / endothelin-1 / non-selective cation channels / voltage-dependent Ltype / trachoal smooth muscle Ca^<2+> current / Ca^<2+>-activated Cl^- current
Research Abstract	1)大動脈血管平滑筋細胞において、vasopressinやendothelin-1などのagonistは、Ca^<2+>透過性陽イオンチャネルを活性化し、細胞外からのCa^<2+>流入をきたし、これが収縮に重要な役割を演じていることが明らかとなった(Nakajima et al.Heart Vessels 9:65-70,1995;Nakajima et al.,Journal of Molecular Cellular Cardiology(in press,1996))。又、近年、estrogenの補充療法が心血管系の死亡率を有意に低下させることが、報告されているが、その機序については不明であった。我々は、estrogenのこのCa^<2+>透過性陽イオンチャネルに対する作用につき検討するとともに、estrogenは、電位依存性L型Ca^<2+>チャネルを抑制することにより血管のtonusを調節していることを明らかにした(Nakajima et al.European Journal of Pharmacology 294:625-635,1995)。 2)気管平滑筋において、Ca^<2+>依存性Cl^-電流が、neurokininなどのagonist刺激や電位依存性L型Ca^<2+>電流の活性化による細胞外からのCa^<2+>流入により活性化され、その機序につき明らかにするとともに、細胞膜電位の脱分極と活動電位持続時間に明らかな影響をきたすことが、現在までの検討で明らかになった(Nakajima et al.,Pflugers Arch 430:552-562,1995;Hazama et al.,Journal of Physiology(London),in press),1996)。

Research Products
(8 results)

All Other

All Publications (8 results)

[Publications] T.Nakajima et al.: "lonic basis of neurokinii A-Indaced depolarijaction in single smooth muscle calls isolated from the guinea-pigtrackea" Pfliigers Arch. 430. 552-562 (1995)
[Publications] T.Nakajima et al.: "Mecharism of vasopressin-induced calcuim inflex in aortic smooth muscle calls:hnooloement of calcuim-pornoalle non-selethie claon channel" Heart Vessels. 9. 65-70 (1995)
[Publications] T.Yamashita et al.: "Flecainide inhibits the transient outward current in atrial myooytes isolated from the rabbit heart" The journal of pharmacology and EXperimentel the lopeatics. 274. 315-321 (1995)
[Publications] T.Yamashita et al.: "Regional differences in transient outward ourrent density and inhomoyenecties repolarigation in rabbit ryht atriun" Circulation. 92. 3061-3069 (1995)
[Publications] T.Nakajima et al.: "17β-estradiol inhibits the voltage-dyendent L-type Ca^<2+> currents in aortic ssnooth muscle cells" European Journal of Pharmacology. 294. 625-635 (1995)
[Publications] T.Nakajima et al.: "Auditory stimuli as a mojor cause of syncope in a jratcent with idiopathc long OT syndrone" Japanese aiculation Journal. 59. 241-246 (1995)
[Publications] T.Nakajima et al.: "Endothelis-1 and vasopressis activate Ca^<2+>-permeable nen selective cation channels is aortic smooth myscle cells:Mechanism of receptor-mediated Ca^<2+>-inflwx" Journal of Molecular and cellulan cardiology. (in press). (1996)
[Publications] H.Hazama et al.: "Neurokinin A and Ca^<2+> current adisate Ca^<2+>-activateel Cl^- currents in gwneo-pig tracheal myo oytes" Journal of Physiology. (in press). (1996)

1995 Fiscal Year Annual Research Report

血管平滑筋のイオンチャネルの機能とその制御機構に関する研究

Principal Investigator

中島 敏明 東京大学, 医学部(病), 助手 (50227790)

Research Products

[Publications] T.Nakajima et al.: "lonic basis of neurokinii A-Indaced depolarijaction in single smooth muscle calls isolated from the guinea-pigtrackea" Pfliigers Arch. 430. 552-562 (1995)

[Publications] T.Nakajima et al.: "Mecharism of vasopressin-induced calcuim inflex in aortic smooth muscle calls:hnooloement of calcuim-pornoalle non-selethie claon channel" Heart Vessels. 9. 65-70 (1995)

[Publications] T.Yamashita et al.: "Flecainide inhibits the transient outward current in atrial myooytes isolated from the rabbit heart" The journal of pharmacology and EXperimentel the lopeatics. 274. 315-321 (1995)

[Publications] T.Yamashita et al.: "Regional differences in transient outward ourrent density and inhomoyenecties repolarigation in rabbit ryht atriun" Circulation. 92. 3061-3069 (1995)

[Publications] T.Nakajima et al.: "17β-estradiol inhibits the voltage-dyendent L-type Ca^<2+> currents in aortic ssnooth muscle cells" European Journal of Pharmacology. 294. 625-635 (1995)

[Publications] T.Nakajima et al.: "Auditory stimuli as a mojor cause of syncope in a jratcent with idiopathc long OT syndrone" Japanese aiculation Journal. 59. 241-246 (1995)

[Publications] T.Nakajima et al.: "Endothelis-1 and vasopressis activate Ca^<2+>-permeable nen selective cation channels is aortic smooth myscle cells:Mechanism of receptor-mediated Ca^<2+>-inflwx" Journal of Molecular and cellulan cardiology. (in press). (1996)

[Publications] H.Hazama et al.: "Neurokinin A and Ca^<2+> current adisate Ca^<2+>-activateel Cl^- currents in gwneo-pig tracheal myo oytes" Journal of Physiology. (in press). (1996)

中島敏明東京大学, 医学部(病), 助手 (50227790)