• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1996 Fiscal Year Final Research Report Summary

The effect of fatty acids in mucosal immunity

Research Project

Project/Area Number 07670897
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionJuntendo University School of Medicine

Principal Investigator

YAMASHIRO Yuichiro  Juntendo Univ.School of Med., Dept.of Pediatrics, Ass.Professor, 医学部, 助教授 (10053159)

Co-Investigator(Kenkyū-buntansha) OGUCHI Satoshi  Juntendo Univ.School of Med., Dept.of Pediatrics, Assistant, 医学部, 助手 (30214092)
SHIMIZU Toshiaki  Juntendo Univ.School of Med., Dept.of Pediatrics, Lecturer, 医学部, 講師 (30260889)
Project Period (FY) 1995 – 1996
Keywordsanaphylactic type allergy / food-sensitive enteropathy / ulcerative colitis / n-6 fatty acids / n-3 fatty acids / low-fat diet / leukotriene B4 / leukotriene C4
Research Abstract

In order to clarify the mechanism of delayd- and early-type hypersensitive reactions in intestine, as well as the ulcerative colitis, we generated these animal models and exapmined the morphological and immunological reactions in these models. We have also investigated whether a change of dietary fatty acid composition affects leukotriene synthesis and mucosal damage in the intestine by using these models fed with specially designed diets prepared from soybean oil, perilla oil, corn oil, and 1/8th volume of corn oil (low fat diet).
Delayd-type hypersensitive mice model. The model was prepared by feeding ovalbumin to BALB/c mice after intraperitoneal injection of cyclophosphamide. Villous atrophy, crypt hyperplasia, so called mucosal damage, and increased numbers of intraepithelial lymphocytes (IELs) were confirmed in the antigen-challenged hypersensitive mice as seen in food-sensitive enteropathy in humnas. In these mice, we found that IELs and LPLs, which are CD4- and CD8-positive cell … More s as well as TCR-alpha/beta-positive cells that have migrated from peripheral blood, are activated by orally administered antigens and cause mucosal damage in the food-sensitive enteropathy. We have also found that reducing cell membrane levels of n-6 fatty acids by feeding less n-6 fatty acids or supplementing n-3 fatty acids by feeding mice model with specially designed diets, is important to suppress leukotriene biosynthesis for prevention from mucosal damage in food-sensitive enteropathy.
IgE-mediated hypersensitive mice model. A mouse model was generated by subcutaneous implantation of the murine hybridoma producing monoclonal anti-TNP IgE antibody. In these mice model, diarrhea, mucosal edema, and a decrease of subcutaneous blood flow were observed after the antigen challenge. An increase in occurrence of mast cells and IgE-bearing cells in the lamina propria of the intestinal mucosa and a substantial increase in serum histamine level were observed after the antigen challenge. These findings suggested that oral administration of the antigen actually induced anaphylactic shock in our mouse model. This reaction was most likely to be mediated by mast cell activation, in response to the IgE-antigen complex. We have also found that the perilla-oil diet and low-fat diet were effective to prevent intestinal mucosa from these reactions.
Ulcerative colitis rat model. A rat model was generated by feeding rats with dextran-sulfate sodium. Diarrhea, bloody stool and crypt abscess were confirmed in these rats. Leukotriene production and the decrease of mucosal blood flow was prevented by feeding these rats with perilla-oil diet. We have also found that it was more effective with vitamin E and could keep rats free from mucosal damage. Less

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 大塚 宜一: "マウス消化管における過敏反応の免疫病理学的検討 第2報" 日本小児科学会雑誌. 99. 771-776 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大塚 宜一,羅 智靖: "特集:食物アレルギー“即時型アレルギー効果相の実験モデル"" アレルギーの領域. 2. 605-609 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大塚 宜一,山城 雄一郎: "粘膜免疫と食物アレルギー" 喘息. 8. 49-55 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohtsuka Y,Yamashiro Y: "Food antigen activate intraepithelial and lamina propria lymphocytes in food-sensitive enteropathy" Pediatric Research. 39. 862-866 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohtsuka Y,Yamashiro Y: "Induction of anaphylactic shock in mice by orally administered antigen and its prevention with soluble high affinity reaeptor for IgE" J Allergy Clin Immunol. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大塚 宜一,羅 智靖: "特集:食物アレルギー;食物アレルギーの効果相モデル" アレルギー科. 3. 152-159 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大塚 宜一,山城 雄一郎: "サイトカインとアレルギー" 茆原順一、宮坂信之編集,メデイカルレビユ-社, 255 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大塚 宜一,山城 雄一郎: "食品アレルギー対策ハンドブック" 上野川修一、近藤直実編集,サイエンスフォーラム社, 491 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohtsuka Y,Yamashiro Y,Maeda M,Oguchi S,Shimizu T,Nagata S,Yagita H,Yabuta K,Okumura K.: "Food antigen activate intraepithelial and lamina propria lymphocytes in food-sensitive enteropathy in mice." Pediatric Research. 39. 862-866 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohtsuka Y,Naito K,Yamashiro Y,Yabuta K,Okumura K,Ra C.: "Induction of anaphlactic shock in mice by orally administered antigen and its prevention with soluble high affinity receptor for IgE." J Allergy Clin Immunol.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi