1997 Fiscal Year Final Research Report Summary
Neuropeptides and phorbolester-induced skin tumor promotion
| Project/Area Number |
07670960
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | OSAKA CITY UNIVERSITY |
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Principal Investigator |
KONO Takeshi Osaka City University, Medical school, Lecturer, 医学部, 講師 (50170197)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUNO Nobuyuki Osaka City University, Medical school, Lecturer, 医学部, 講師 (80271188)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Tumor promotion / Neuropeptides / carcinogenesis |
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| Research Abstract |
We investigated relations of some neuropeptides (NP) and tumor promotion process in the skin. NPs used were substance P (SP), calcitonin gene-related peptide (CGRP), vasoactive intestinal peptide (VIP), nerve growth factor (NGF), beta-endorphine (beta-EP) and somatostatin (SOM). As a tumor promotional stimulation. 12-o-tetradecanoypholbol-13-acetate (TPA) was applied to the guinea pig skin.
TPA treatment had no effect on tissue or blood concentration of CGRP,VIP,NGF,SOM.SP concentation increased in the tissue in 6 hr after TPA treatment, and in the blood within 12 hr. beta-EP concentration increased only in the blood in 6 hr.
As a next step, interrelations between NPs and ornithine decarboxylase (ODC), a marker enzyme for tumor promotion process, were investigated. NGF or beta-EP had no effect on TPA-induced ODC activity. On the other hand, SP,CGRP and SOM suppressed TPA-induced ODC activity by about 60% at 3,6 and 9 hrs after TPA treatment. VIP showed biphasic action. VIP pre-treatment stimulated ODC activity, but VIP post-treatment suppressed ODC activity. Any NP had no effect on ODC mRNA level and TPA-stimulated keratinocyte proliferation in tissue section.
These results suggest interactions between TPA-induced tumor promotional process in the skin and NPs.
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