Analysis of Anti-Carbonic Anhydrase Antibody

1996 Fiscal Year Final Research Report Summary

• Project/Area Number: 07670974
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: KAWASAKI MEDICAL SCHOOL
• Principal Investigator: UEKI Hiroaki Kawasaki Medical School School of Medicine, Professor, 医学部, 教授 (30069017)
• Co-Investigator(Kenkyū-buntansha): OMORI Kentaro Kawasaki Medical School School of Medicine, Faculty assistant, 医学部, 助手 (20278930) ; WATANABE Keisuke Kawasaki Medical School School of Medicine, Faculty assistant, 医学部, 助手 (80268606) ; ONO Mariko Kawasaki Medical School School of Medicine, Faculty assistant, 医学部, 助手 (70289180) ; ONO Masashi Kawasaki Medical School School of Medicine, Faculty assistant, 医学部, 助手 (90278929)
• Project Period (FY): 1995 – 1996
• Keywords: Rheumatic Autoimmune Disease / Carbonic Anhydrase / Autoantibody

Research Abstract

At first, using immunoblot and ELISA,the incidence of anti-carbonic anlydrase (CA) antibodies in Rheumatic autoimmune disease (RAD) was investigated. Anti-CA antibodies were found in 20-30% of sera from patients with RAD.All anti-CA sera were reactive with both human CAI and CAII.The correlation of it with clinical syndrome has been investigated. Second, epitopes on CAI and CAII antigen which recognized by RAD sera or natural autoantibody found in healthy individuals were analyzed using synthesized peptides. Anti-CA antibodies in RAD patients recognized alfa-helical portion on amino-terminus of CA and active domains of CA enzyme. This may evident the inhibitory effect of anti-CA antibodies against CA activity which previously discussed. In contrast, natural autoantibodies exclusively reacted with a portion located at carboxy-terminus of CA.These observation may suggest the distinguished mechanism of autoimmunity in RAD.We also reported a patient with anti-CA antibody who has similar symptoms to CA deficiency.