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1996 Fiscal Year Final Research Report Summary

"Study of early radiation-induced cell death using two cell lines with different radiosensitivities in vitro"

Research Project

Project/Area Number 07670983
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionGunma University School of Medicine

Principal Investigator

MITSUHASHI Norio  Department of Radiology and Radiation Oncology Gunma University School of Medicine, 医学部, 助教授 (20008585)

Project Period (FY) 1995 – 1996
KeywordsRadiosensitivity / Radiation-induced apoptosis / Cisplatin / Interphase death / Thermosensitivity / Synergistic effect / Rat yolk sac tumor
Research Abstract

A radiosensitive rat yolk sac tumor cell line, NMT-1, and A radioresistant variant cell line, NMT-1R were used in this study to investigate the difference of radiosensitivity, chemosensitivity and thermosensitivity.
1.For NMT-1R,Do for radiation sensitivity was 165 cGy, 1.7 times as large as for NMT-1. The extrapolation number, n, was 1.48 for NMT-1R although that for NMT-1 was 1.8.
2.Although a clear G2 delay was observed after irradiation with a dose of 2 Gy for both cell lines, NMT-1R had a shoreter recovery time from G2 block than NMT-1. G1 arrest was observed in NMT-1.
3.NMT-1 showed much higher incidence of early morphological changes, especially apoptosis, after irradiation with more than 5 Gy compared with NMT-1R cell.
4.Development of apoptosis in NMT-1 was observed as a function of time within 24 h after irradiation with a dose of 5 Gy. The incidence of apoptosis reached a plateau with a single dose of 5 Gy.
5.NMT-1 was 4 times as sensitive to cisplatin as NMT-1R based on ID_<50>.
6.Cisplatin had a synergistic effect by a factor of 1.30-1.39, which was independent of the time-course and sequence in combination with radiation within 6 h on both cell lines.
7.The "TO" for heat sensitivity was 2.5 h at temperature of 42 ゚C for NMT-1 but that for NMT-1R was 0.7 h. NMT-1R was more sensitive to heat than NMT-1.
8.Thermal tolerance ratios for NMT-1 and NMT-1R at 6 hr interval was 8.09 and 6.28 respectively. HSP70 expression was induced predominatly on NMT-1 cells following heating.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Mitsuhashi,N.: "A radiosesistant vuiant cell line,NMF1R,isolated from a radiosensitive rat yolk sac human cell line. NMF1 : differences of early radiation-induced perpelolgicalchanges,especially apptosis" Int.J.Radiat.Biol.69(3). 329-336 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamoto,S.: "An interaction of cisplatine and radiation in two rat yolk sac tumau cell lines with different radiosensitivities in vitro" Int.J.Radiat.Biol.70(6). 747-753 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakurai,H.: "Enhanced cytotoxicety in combination of low dose-rate inadiation with hyperthermia in vitro" Int.J.Hyperthermia. 12(3). 355-366 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ebara,T.: "Prognostic significance of immuohistochemically detected P53 protein expression in stage IIIB squamono cell auinoma of the uterine cerix" Gyne.Oncol.63(2). 216-218 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hasegawa,M.: "P53 protein expression and radiation-induced apoptosis in human Aumors transplanted to nude mice" Radial.Med.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furuta,M.: "A rapidlly increased accumulation of FDG in an iaoulated human Aumor Xewgraft" Eur.J.Nucl.Med.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furuta M,Hasegawa M,Mitsuhashi N,Yamakawa M and Niibe H.: "Sonographic-histopathologic correlation on human tumor in nude mice treated with percutaneous ethanol injection therapy and radiation therapy." Invest.Radiol. 30(1). 10-20 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuhashi N,Takahashi T,Sakurai H,Yamakawa M,Hasegawa M,Furuta M,Matsumoto H,Higuchi K,Hayakawa K and Niibe H.: "Estblishment and characterization of a new human lung poorly differentiated adenocarcinoma cell line, GLL-1, producing carcinoembryonic antigen (CEA) and CA19-9." Lung Cancer. 12(1,2). 13-24 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hasegawa M,Niibe H,Mitsuhashi N,Yamakawa M,Kato S,Furuta M,Kazumoto T, Hayakawa K and Nakazato Y.: "Hyperfractionated and hypofractionated radiation therapy for human malignant glioma xenograft in nude mice.19GC03 : Jpn.J.Cancer Res" 86(9). 879-884 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi T,Mitsuhashi N,Sakurai H and Niibe H.: "Modifications of tumor-associated antigen expression on human lung cancer cells by hyperthermia and cytokine." Anticancer Res. 15(6B). 2601-2506 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuhashi N,Takahashi T,Sakurai H,Nozaki M,Akimoto T,Hasegawa M,Saito Y,Matsumoto H,Higuchi K,Maebayashi K and Niibe H.: "A radioresistant cell line, NMT-1R,isolated from a radiosensitive rat yolk sac tumour cell line, NMT-1 : differences of early radiation-induced morphological changes, especially apoptosis." Int.J.Radiat Biol. 69(3). 329-336 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakurai H,Mitsuhashi N,Takahashi T,Hashida I and Niibe H.: "Enhanced cytotoxicity in combination of low doserate irradiation with hyperthermia in vitro." Int.J.Hyperttermia. 12(3). 355-366 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ebara T,Mitsuhashi N,Saito Y,Sakurai H,Hasegawa M,Takahashi T,Hayakawa K and Niibe H.: "Prognostic significance of immunohistochemically detected p53 protein expression in stage IIIB squamous cell carcinoma of the uterine cervix treated with radiation therapy alone." Gynecol. Oncol. 63(2). 216-218 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamoto S,Mitsuhashi N,Takahashi T,Sakurai H and Niibe H.: "An interaction of cisplatin and radiation in two rat yolk sac tumour cell lines with different radiosensitivities in vitro." Int.J.Radiat. Biol. 70(6). 747-753 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hasegawa M,Yamakawa M,Mitsuhashi N,Ohno T,Imai R,Sato R,Shiojima K and Niibe H.: "Relationships between apoptosis and p53 protein expression in squamous cell carcinoma of the esophagus." Ann.Cancer Res.Ther.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hasegawa M,Mitsuhashi N,Yamakawa M,Furuta M,Maebayashi K,Imai R,Hayakawa K and Niibe H.: "p53 protein expression and radiation-induced apoptosis in human tumors transplanted to nude mice" Radiat.Med. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furuta M,Hasegawa M,Mitsuhashi N and Niibe H.: "A rapidly increased accumulation of FDC in an irradiated human tumor xenograft." Eur.J.Nucl.Med. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi T,Mitsuhashi N,Sakurai H,Murata O,Kitamoto Y,Matsumoto H,Higuchi K and Niibe H.: "Enhancement of piralubicin (THP-adriamycin) by mild hyperthermia in vitro." Int.J.Hyperthermia. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hasegawa M,Yamakawa M,Mitsuhashi N,Furuta M,Ohno T,Hayakawa K and Niibe H.: "Tumor radiosensitivity and radiation-induced apoptosis in human tumors transplanted to nude mice. (in Japanese)" J.Jpn.Soc.Ther. 7(1). 47-53 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hasegawa M,Yamakawa M,Mitsuhashi N,Furuta M,Sakurai H,Hayakawa K and Niibe H.: "Immunohistochemical study of radiation-induced apoptosis and oncogenes. (in Japanese)" Nippon Acta Radiologica. 55(11). 53-54 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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