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1996 Fiscal Year Final Research Report Summary

Molecular Biological Approach towards Efficient Radioimmunotherapy

Research Project

Project/Area Number 07671027
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionKeio University School of Medicine

Principal Investigator

NAKAMURA Kayoko  Keio Univ.School of Med.Dept.of Radiol.Assist.Profess., 医学部, 専任講師 (20124480)

Project Period (FY) 1995 – 1996
KeywordsRI-labeled antibody / Radioimmunotherapy / Tumor cells / CEA / Apoptosis / Gastric cancer / Leukemia / I-131
Research Abstract

The aim of this study is to evaluate the tumor cells treated with radiolabeled monoclonal antibodies from the molecular biological point of view. The effects of dose of radiolabeled antibodies and fractionation were investigated to get the following results.
1. Human gastric cancer cells producing CEA were incubated with I-131-labeled anti-CEA monoclonal antibody :
(1) No apoptosis was observed in tumor cells.
(2) Cytotoxicity was dose dependent, and repeated incubation caused higher toxicity than one incubations.
2. Murine leukemia cells were incubated with I-131-labeled monoclonal antibody which was reactive with cell surface antigen :
(1) Apoptosis was observed in tumor cells.
(2) Apoptosis was observed in tumor cells which were incubated with I-131-labeled irrelevant antibody or I-131 ; indicating that apoptosis is not specific for radiolabeled antibodies.
3. Athymic nude mice bearing human gastric cancer xenografts were injected with I-131-labeled anti-CEA monoclonal antibody :
(1) No apoptosis was observed in tumor cells by any dose of I-131-labeled antibody.
(2) Necrosis was observed in xenografts when mice were injected with I-131-labeled antibody intratumorally. Fractionation reduced the uptake of I-131 in the tumors.
4. Hetero, athymic nude, or SCID mice bearing leukemia cells intraperitoneally were injected with I-131-labeled antibody :
(1) Apoptosis was observed in leukemia cells in any mice.
(2) Apoptosis was observed in only hetero mice by the low dose of I-131-antibody. High does of I-131-labeled antibody caused the radiation damage in SCID mice ; indicating that apoptosis occurred through the immunogenic pathways.
5.The study suggested that :
(1) Apoptosis would not be mainly involved in solid tumors by radioimmunotherapy.
(2) Apoptosis is an important factors in hematological tumors in conjunction with the immunogenecity from the host when it is treated by radioimmunotherapy.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Kayoko Nakamura: "Distribution and Pharmacokinetics of In-111-DTPA-IgG in Healthy Human Subjects" RADIOISOTOPES. 44. 303-309 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村佳代子: "酵素阻害剤がヨード標識抗体の生体内分布に及ぼす影響" 免疫・腫瘍核医学. 10. 29-31 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤導直: "In-111標識抗CEAモノクローナル抗体の生体内分布" 腫瘍マーカー研究会誌. 10. 147-149 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kayoko Nakamura: "Effect of Lysosomal Inhibitors on the Fate of I-125-labeled Antibody in Athymic Mice Bearing Human Tumor Xenografts" The Quarterly Journal of Nuclear Medicine. 39. 73 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kayoko Nakamura: "Effect of Lysosomal Inhibitors on Retention of I-125 Activity in Human Tumor Xenografts" Journal of Nuclear Medicine. 36. 159P (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kayoko Nakamura: "Effect of Interferon-minipellet on Radiolabelled Antibody Targeting" European Journal of Nuclear Medcine. 22. 839 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kayoko Nakamura: "Brodistribution of I-125-labeled monoclonal antibody/IL-2 Immunoconjugate in Athymic Mice Bearing Human Tumer Xenograft" TUMOR TARGETING. 2. 153 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村佳代子: "おもしろいバイオ新素材のはなし" 日刊工業新聞社, 265 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kayoko Nakamura: "Distribution and pharmacokinetics of In-111-DTPA-IgG in healthy human subjects." RADIOISOTOPES. 44 (2). 303-309 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kayoko Nakamura: "Effect of enzymatic inhibitors on the biodistribution of I-125-labeled antibody" Immuno-Nuclear Oncology. 10 (2). 29-31 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Michinao Sato: "Biodistribution of In-111-labeled anti-CEA monoclonal antibody" Tumor Markers. 10. 147-149 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kayoko Nakamura: "Effect of lysosomal inhibitors o the fate of I-125-labeled antibody in athymic mice bearing human tumor xenografts." The Quarterly Journal of Nuclear Medicine. 39 (1). 73 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kayoko Nakamura: "Effect of lysosomal inhibitors on retention of I-125-activity in human tumor xenografts." Journal of Nuclear Medicine. 36 (5). 159 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kayoko Nakamura: "Effect of interferon-minipellet on radiolabelled antibody targeting." European Journal of Nuclear Medicine. 22 (8). 839 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kayoko Nakamura: "Biodistribution of I-125-labeled antibody/IL-2 immunoconjugate in athymic mice bearing human tumor xenograft." TUMOR TARGETING. 2 (3). 153 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kayaoko Nakamura: "New Bio-materials"

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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