1996 Fiscal Year Final Research Report Summary
Molecular analysis of anti-inflammatory effect of IL-10 and IL-4 on human monocytes
| Project/Area Number |
07671213
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Kyushu University |
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Principal Investigator |
OTSUKA Takeshi Kyushu University, Faculty of Medicine, assistant professor, 医学部, 助手 (50213773)
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| Co-Investigator(Kenkyū-buntansha) |
IZUHARA Kenji National Institute of Genetics, assistant professor, 助手 (00270463)
TANAKA Yosuke Kyushu University, 医学部, 医員
NEMOTO Yoshiaki Kyushu University, 医学部, 医員
NIIRO Hiroaki Kyushu University, 医学部, 医員
NAKASHIMA Hitoshi Kyushu University, Faculty of Medicine, assistant professor, 医学部, 助手 (70188960)
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| Project Period (FY) |
1995 – 1996
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| Keywords | monocyte / macrophage / interleukin-4 / interleukin-10 / cyclooxygenase / JAK / STAT / signal transduction |
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| Research Abstract |
1.IL-4 suppressed and IL-10 rather enhanced NO by mouse monocytic cell lines. Production of TNF-a or superoxide anions were suppressed by both IL-4 and IL-10, indicating that regulatory mechanism of NO production is not regulated by TNF-a production alone. Macrophage cell lines expressing human IL-4 receptor alpha chain on the cell membrane, were suppressed NO and TNF-alpha by human IL-4.
2.Mouse resident (RPMf) and thioglycollate-elicited (TPMf) peritoneal macrophages released a substantial amount of NO after stimulation with LPS or a combination of LPS and IFN-g (LPS/IFN-g). Both IL-10 and IL-4 inhibitedNO production by stimulated TPMf. On the other hand, NO production by stimulated RPMf was inhibited by IL-4, but was enhanced by IL-10.
3.We examined the activation of STAT molecules by using electrophoretic mobility-shift assayusing (EMSA). Using a DNA probe corresponding to a GAS-motif sequence conserved in the promoter region of cyclooxygenase (COX) -2 genes for human, mouse and rat, STAT5 was shown to be activated in human peripheral monocytes stimulated with LPS.This activation of STAT5 was neutralized partly by an antibody against GM-CSF.
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[Publications] Niiro H,Otsuka T,Izuhara K,Yamaoka K,Ohshima K,Tanabe T,Hara S,Nemoto Y,Tanaka Y,Nakashima H,Niho Y: "Regulation by interleukin-10 and interleukin-4 of cyclooxygenase-2 expression in human neutrophils" Blood. (in press).
Description
「研究成果報告書概要(欧文)」より
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