1996 Fiscal Year Final Research Report Summary
Apoptosis and ischemic neuronal injury in the fetal and neonatal brain
| Project/Area Number |
07671278
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
YOSHIOKA Hiroshi Kyoto Prefectural University of Medicine, Department of Pediatrics, Assistant Professor, 医学部, 講師 (50128724)
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| Project Period (FY) |
1995 – 1996
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| Keywords | fetus / neonate / brain / apoptosis / ischemia / neuronal death |
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| Research Abstract |
In previous experimental studies we have found that hypoxia and/or ischemia often causes karyolexis or picnosis, or neuronal shrinkage, in neonatal brains. In this study, therefore, to know whether such a neuronal change is necrosis or apoptosis, I investigated neuronal changes in neonatal rat model of transient cerebral ischemia (J Cereb Blood Flow Metab 16 ; 237,1996) by TUNEL method or by electron microscopy. Twenty-four hours after 2-hour ischemic event, many neurons in the right sensorimotor cortex were found to be damaged by H & E examination, and about 80% of them were TUNEL positive. Electron microscopic study revealed that chromatin in such cells were devided into small pieces and found along the nuclear membrane. The surface of the pieces was usually irregular. From such electron microscopic findings I concluded that this neuronal change in neonatal rat brain is necrosis, not apoptosis.
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