• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1996 Fiscal Year Final Research Report Summary

Apoptosis and ischemic neuronal injury in the fetal and neonatal brain

Research Project

Project/Area Number 07671278
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Embryonic/Neonatal medicine
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

YOSHIOKA Hiroshi  Kyoto Prefectural University of Medicine, Department of Pediatrics, Assistant Professor, 医学部, 講師 (50128724)

Project Period (FY) 1995 – 1996
Keywordsfetus / neonate / brain / apoptosis / ischemia / neuronal death
Research Abstract

In previous experimental studies we have found that hypoxia and/or ischemia often causes karyolexis or picnosis, or neuronal shrinkage, in neonatal brains. In this study, therefore, to know whether such a neuronal change is necrosis or apoptosis, I investigated neuronal changes in neonatal rat model of transient cerebral ischemia (J Cereb Blood Flow Metab 16 ; 237,1996) by TUNEL method or by electron microscopy. Twenty-four hours after 2-hour ischemic event, many neurons in the right sensorimotor cortex were found to be damaged by H & E examination, and about 80% of them were TUNEL positive. Electron microscopic study revealed that chromatin in such cells were devided into small pieces and found along the nuclear membrane. The surface of the pieces was usually irregular. From such electron microscopic findings I concluded that this neuronal change in neonatal rat brain is necrosis, not apoptosis.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 吉岡 博: "MRS診断と新生児への応用" 周産期医学. 25. (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuto Mitsufuji: "Intracellular alkalosis during hypoxia in newbora mouse brain in the presence of systemic acidosis:aphasphorus magnetic resonance spectroscopic study" Brain & Development. 17. 256-60 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Yoshioka: "Effects of Early Undernitrition and Subsequent Natritional Rehabilitation on Brain Development" Developmental Brain Dysduuction. 8. 66-12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Yoshioka: "Effects of hypercapnia on ECoG and oxidative metabolism in neonatal dog brain" Journal of Applied Physiology. 78(6). 2272-2278 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuto Mitsufuji: "A New Model of Transient Cerebral Ischemia in Neonatal Rats" Journal of Cerebral Blood Flow and Metabolism. 16. 237-243 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Yoshioka: "Seizure Susceptibility During Recovery From Hypercapnia in Neonatal Dogs" PEDIATRIC NEUROLOGY. 15(1). 36-40 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsufuji N,et al: "Intracellular alkalosis during hypoxia in newborn mouse brain in the presence of systemic acidosis : a phosphorus magnetic resonance spectroscopic study." Brain and Development. 17. 256-260 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshioka H,et al: "Effects of hypercapnia on ECoG and oxidative metabolism in neonatal dog brain." Journal of Applied Physiology. 78 (6). 2272-2278 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshioka H,et al: "Effects of early undernutrition and subsequent nutritional rehabilitation on brain development. I.the most critical period." Developmental Brain Dysfunction. 8 (1). 66-72 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsufuji N,et al: "A new model of transient cerebral ischemia in neonatal rats." J Cereb Blood Flow Metab. 16. 237-243 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshioka H,et al: "Seizure susceptibility during recovery from hypercapnia in neonatal dogs." Pediatr Neurol. 15. 36-40 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi