Bone formation by the implantation of bone marrow cells

1996 Fiscal Year Final Research Report Summary

• Project/Area Number: 07671283
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Hokkaido University
• Principal Investigator: MIZUNO Morimichi Hokkaido Univ., Sch.of Dent., Inst., 歯学部, 助手 (10125354)
• Co-Investigator(Kenkyū-buntansha): KUBOKI Yoshinori Hokkaido Univ., Sch.of Dent., Prof., 歯学部, 教授 (00014001)
• Project Period (FY): 1995 – 1996
• Keywords: Osteoblasts / Bone / Bone marrow cells / Collagen gel

Research Abstract

In this study, we attempted to form a mass of bone by the implantation of osteoblastic cells differentiated from bone marrow stromal cells. Dexamethasone or vitamin C have been utilized to induce the osteoblastic differentiation so far, however, there is no report that these cells formed bone in vivo. Recently we found that collagen gel induced the osteoblastic differentiation without dexamethasone or vitamin C.These cells with collagen gel induced bone and a volume of bone increased time-dependently in vivo. On the other hand, un-differentiated bone marrow cells with collagen gel did not form bone. These results indicate that bone marrow stromal cells which expressed the osteoblastic phenotypes could form bone effectively in vivo. The effect of collagen gel is specific for type I,and type II-V collagen did no? possess the inducing activity. Furthermore, TGF-beta enhanced the osteoblastic differentiation by collagen gel. Our results demonstrate that bone marrow cells could form a mass of bone by our procedure. The benefit of our method is 1) we do not use dexamethasone, which has a potent side-effect, 2) we utilized collagen, which shows affinity to tissues.

Research Products

• [Publications] Mizuno M.: "Osteogenesis by Bone Marrow Stomal Cells Mainbained on Type I Collagen Matrix Gels In Vivo" Bone. 20. 101-107 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mizuno M.: "The osteoblastic MC3T3-E1 cells synthesized C-terminal propeptide of type I collagen, which promoted cell-atbachment of osfeoblasi" Biochenica et Biophysica Acba. 1310. 97-102 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mizuno M.: "Bone chondroad herin promobes allachment of osbeoblastic cells to solid-stabe subsbrates and shows affiniby to collagen" Calcified Tissne Inbernabional. 59. 163-167 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mizuno, M., Kitajima, T., Tomita, M., Kuboki, Y.: "The osteoblastic MC3T3-E1 cells synthesized C-terminal propeptide of type I collagen, which promoted cell-attachment of osteoblasts." Biochemica et Biochysica acta. 1310. 97-102 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mizuno, M., Fujisawa, F., Kuboki, Y.: "Bone chondroadherin promotes attachment of osteoblastic cells to solid-state substrates and affinity to collagen." Calcified Tissue International. 59. 163-167 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mizuno, M., Shindo, M., Kobayashi, D., Tsuruga, E., Amemiya, A., Kuboki, Y.: "Osteogenesis by bone marrow stromal cells maintained on type I collagen matrix gels" Bone. 20. 101-107 (1997)
  • Description: 「研究成果報告書概要(欧文)」より