1997 Fiscal Year Final Research Report Summary
Insulin-like growth factors (IGFs). their receptors, and IGF binding proteins in human endocrine cancer
| Project/Area Number |
07671289
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | University of Tsukuba |
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Principal Investigator |
YASHIRO Tohru University of Tsukuba, Institute of Clinical Medicine, Assistant Professor, 臨床医学系, 講師 (20157978)
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| Co-Investigator(Kenkyū-buntansha) |
HARA Hisato University of Tsukuba, Institute of Clinical Medicine, Assistant Professor, 臨床医学系, 講師 (80189688)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Thyroid cancer / IGF-I / ICFBP |
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| Research Abstract |
We investigated the tissue contents of immunoreactive insulin-like growthfactor-I (irIGF-I) and IGF binding proteins (IGFBPs) in human thyroid carcinoma and compared them with those of normal thyroid tissue. when irIGF-I was measured after separation of the IGFBPs by gel-filtration. its content in carcinoma tissue was not different from that in adjacent normal tissue. Nor was there any difference in the abundance of IGF-I mRNA expression determined by slot blot analysis. IGFBPs activity measured in terms of ^<125>I-IGF-I binding was significantly higher in carcinoma extracts. Western ligand blot analysis of IGFBPs revealed that 30 to 34 Kd binding proteins are more prominent in carcinoma tissue than in normal adjacent tissue. From the molecular weight of the protein, it can be estimated as IGFBP-1(30Kd) or IGFBP-2(34Kd).Northern blot analysis demonstrated that IGFBP-2 mRNA levels are higher in carcinoma tissue than that in normal tissue but we could not detect IGFBP-1 mRNA in the thyroid tissur by total RNA.Since IGFBPs have been reported to modulate cellular responses to IGF-1, the present data suggest that the IGFBP-2 overexpression in carcinoma tissue is involved in regulating growth of human thyroid cancer cells.
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