1996 Fiscal Year Final Research Report Summary
Boneficial Effect of Kupffer cell blochade on Preservation Liver Enjury
| Project/Area Number |
07671311
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KAMEI Takafumi Faculty of Medicine KYUSHU UNIVERSITY Lecture, 医学部, 講師 (30243942)
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| Project Period (FY) |
1995 – 1996
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| Keywords | Reperfusion Injury / kupffer cell blockade / Hepatectomy / 肝切除 |
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| Research Abstract |
We have investigated the effect of Kupffer cell blockade GdCl3 on liver function in warm ischemic model in rats. Lateral and central liver lobes were made ischemic for 70 min.by selective clamping of the hepatic artery and portal vein. Preoperative treatment with Kupffer cell blockade GdCl3 significantly reduced Kupffer cell TNF production and serum GPT levels compared to those of control untreated group. The other experiment was carried out to clarify the effect of Kupffer cell blockade GdCl3 on transplantation of cold preserved liver. Untreated or Kupffer cell blockade GdCl3 trated donr rat liver was hypothermically preserved for 12 hrs before transplantation. Kupffer cell blockade GdCl3 administration dramatically improved survival of the recipients transplanted with cold preserved liver. 70% of the recipients survived for more than 7 days in Kupffer cell blockade GdCl3 group while all the recipients died within 1 day unreated group. Kupffer cell TNF prodution was significantly reduced in GdCl3 pretreated liver compared to control liver.
These findings clearly indicate that Kupffer cell activation plays an important role in cold and warm ischemic insult in rat liver. Kupffer cell blockade GdCl3 might be a new therapeutic procedure to reduce these ischemic change in the liver.
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