1997 Fiscal Year Final Research Report Summary
Analysis for biological function of carbohydrate antigens in breast cancer, and its application to clinical medicine
| Project/Area Number |
07671349
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Aichi Cancer Center |
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Principal Investigator |
NARITA Tatsuhiko Laboratory of Experimental Pathology, Aichi Cancer Center, Investigator, 病理学第二部, 主任研究員 (40270714)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAGI Hiroshi Department of Surgery II,Nagoya University, Professor, 医学部・外科学第二講座, 教授 (70154755)
KANNNAGI Reiji Laboratory of Experimental Pathology, Aichi Cancer Center, Chief, 病理学第二部, 部長 (80161389)
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| Project Period (FY) |
1995 – 1997
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| Keywords | Breast cancer / Cell adhesion molecules / Carbohydrate antigen / E-selectin |
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| Research Abstract |
We investivated the biological function of carbohydrate antigens in breast cancer, and vertificated it by using clinical materials. Sialy Lewis^x antigen (sLeX) was expressed commonly in cell lines derived from human breast cancer. The adhesion of cancer cells to endothelial cells was mediated by sLeX via E-selectin. Fucosyltransferase IV is chiefly involved in syntheses of sLeX on cancer cells. The breast cancer cells as IL-beta, from lymphocytes. Following the initial adhesion mediated by sLeX via E-selectin, breast cancer cells recieve stimulation from cytokines, such as HB-EGF and HGF,produced by endothelial cells and transmigrate to extravascular tissues through the augmentation of integrins. E-selectin was expressed on the endothelial cells of the small vessels adjacent to the cancer nests in breast cancer tissues. The concentrations of soluble E-selectin, sLeX and HGF were significantly elevated in the sera of patients with advanced and recurrent breast cancer. The prognosis of patients in whom sLeX was expressed in their cancerous tissues, was significantly poorer than in patients in whom it was negative. Consequently, we clarfied the mechanism of adhesion of breast cacner cells to endothelial cells. It has also been sugested that adhesion molecules are useful in clinical medicine of breast cancer as tumor marker and prognostic indicator.
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