1996 Fiscal Year Final Research Report Summary
Clinical significance of serum delta bilirubin during obstructive jandice.
| Project/Area Number |
07671406
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
CHIJIIWA Kazuo Kyushu Univ.Fac.Med lecturer, 医学部, 講師 (90179945)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAMEOKA Nubuhisa Kyushu Univ.Fac.Med research fellow, 医学部, 医員
SHIMIZU Shuji Kyushu Univ.Fac.Med assistant Prof., 医学部, 助手 (70274454)
KUROKI Syoji Kyushu Univ.Fac.Med assistant Prof., 医学部, 助手 (30215090)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Keywords | delta-bilirubin / obstructive jaundice / biliary drainage |
|---|
| Research Abstract |
delta-Bilirubin, a nonenzymatic covalently bound complex with albumin, is nonenzymatically formed in serum and has a long half-life irrespective of hepatorenal function. The aim was to examine if delta-bilirubin reflects the duration of obstructive jaundice and efficacy of biliary drainage. Obstructive jaundice was developed in 17 dogs either for 4 (short term ; n=9) or 11 (long term ; n=8) days and then external biliary drainage was performed. Serum total, direct, and delta-bilirubin fractions were analyzed by high-performance liquid chromatography. delta-Bilirubin was not detectable in serum before biliary obstruction. The serum total and derect bilirubin levels increased rapidly and reached a plateau within 2 days after the biliary obstruction, whereas the concentration and proportion of delta-bilirubin gradually increased and significantly correlated with the duration of obstructive jaundice. Before biliary drainage, the proportion of delta-bilirubin was 28.9(]SY.+-。[)2.4% in the shortterm and 42.5(]SY.+-。[)2.4% in the long-term obstruction group. The decline indices of serum total, direct, and total minus delta-bilirubin in the short-term group (-0.17, -0.19, and -0.26, respectively) were significantly (P<0.05) greater than those in the long-term obstruction group (-0.10, -0.10, and -0.13). The decline index of delta-bilirubin itself was similar between the groups. In conclusion, the concentration and proportion of delta-bilirubin in serum reflect the duration of jaundice. Because delta-bilirubin is not excreted into bile and urine and is not toxic to organs, a decline index of total bilirubin minus delta-bilirubin, the excretable bilirubin fraction might be better to assess the efficacy of biliary drainge. Similar results were obtained from 21 human samples.
|
