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1996 Fiscal Year Final Research Report Summary

Investigation of the metastatic mechanism by a new in vitro invasion asseay using monolayrs of vascular endothelial cells.

Research Project

Project/Area Number 07671441
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionUniversity of Occupational and Environmental Health

Principal Investigator

ITOH Hideaki  University of Occupational and Environmental Health, Shool of Medicine, Professor, 医学部, 教授 (90038852)

Co-Investigator(Kenkyū-buntansha) 平田 敬治  産業医科大学, 医学部, 助手 (70269059)
NAKAYAMA Yoshifumi  University of Occupational and Environmental Health, Shool of Medicine, Research, 医学部, 助手 (50279337)
Project Period (FY) 1995 – 1996
Keywordsinvasion assay / endothelial cell / metastasis / plasminogen activator / matrix metalloproteinase / c-met / Siaryl Lewis^a / E-cadherin
Research Abstract

Metastasis is a complex process. The interaction between endothelial cells and cancer cells is very important in this process. We established an in vitro invasion model using monolayrs of endothelial cells for investigation of this interaction. In human renal cancer cell lines, highly metastatic cell lines were more invasive than their low metastatic counterpart in our in vitro model. Our in vitro invasion assay using calf pulumonary arterial endothelial cells would be useful to evaluate protease activities and colony formation during invasion. Further examination of other sets of low and highly metastatic carcinoma cell lines is required to evaluate this in vitro invasion assay system. Now, we are producing several cell lines which will be useful for our invasion assay. In human renal cancer cell lines, we indicated the increased mRNA expressions of t-PA,u-PA and c-met by morthern blot analysis. In human colon carcinoma cell lines, we indicated the increased mRNA expressions of u-PA,MMP-2 and c-met by northern blot analysis, and we also indicated the increased expression of Siaryl Lewis^a and decreased expression of E-cadherin at cell surface by flow-cytometric analysis. These results were reported at some conferences and papors.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 中山善文、後信、他: "血管新生と転移" Molecular medicine. 32. 406-413 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Takeda,K.Hisatomi,et.al.: "A 10-year survivior with unresectable hepatic matastases from sigmoid colon carcinoma treated with regional chemotherapy." Jpn. J. Surg. (Surgery Today). 25. 440-443 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Katoh,N.Nagata,et.al.: "Glutathione S-transferase M1 (GSTM1) and T1 (GSTT1) genetic polymorphism and susceptibility to gastric and colorectal adenocarcinoma." Carcinogenesis. 17. 1855-1859 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Nakayama,S.Naito,et.al.: "An in vitro invasion model for human renal cell carcinoma cell lines mimicking their metastatic abilities." Clin. Exp. Metastasis. 14. 466-474 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ueki,Y.Nakayama,et.al.: "Significance of the expression of proliferation-associated nucleolar antigen p120 in human colorectal tumors." HUMAN PATHOLOGY. 28. 74-79 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Ryuto,S.Jimi,et.al.: "Inhibition by all-trance-retinoic acid of invasion and phosphorylation of β-catenin tyrosine in metastatic human renal carcinoma cells." Cancer Res. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayama, Y., Ushiro, S., et. al.: "New-born blood vessels and metastasis." Molecular medicine. 32 (4). 406-413 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeda, S., Hisatomi K., et. al.: "A 10-year survivior with unresectable hepatic matastases from sigmoid colon carcinoma treated with regional chemotherapy." Jpn. J.Surg. (Surgery Today). 25 (5). 440-443 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katoh, T., Nagata, N., et. al.: "Glutathione S-transferase M1 (GSTM1) and T1 (GSTT1) genetic polymorphism and susceptibility to gastric and colorectal adenocarcinoma." Carcinogenesis. 17 (9). 1855-1859 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayama, Y., Naito, S., et. al.: "An in vitro invasion model for human renal cell carcinoma cell lines mimicking their metastatic abilities." Clin. Exp. Metastasis. 14 (10). 466-474 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueki, T.Nakayama, Y.et. al.: "Significance of the expression of proliferation-associated uncleolar antigen p120 in human colorectal tumors." HUMAN PATHOLOGY. 28 (1). 74-79 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ryuto, M., Jiji, S.et. al.: "Inhibition by all-trance-retinoic acid of invasion and phosphorylation of beta-catenin tyrosine in metastatic human renal carcinoma cells." Cancer Res. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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